Diabetic Wound Healing
Diabetic Wound Healing - Related Videos
05:39
Murine Model of Wound Healing
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Cited by 207 •
2013
A murine model of cutaneous wound healing that can be used to assess therapeutic compounds in physiological and pathophysiological settings.
11:13
Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells
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Cited by 27 •
2016
An example of adenoviral gene therapy in the human diabetic organ-cultured corneas is presented towards the normalization of delayed wound healing and markedly reduced epithelial stem cell marker expression in these corneas. It also describes the optimization of this process in stem cell-enriched limbal epithelial cultures.
09:15
Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model
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Cited by 8 •
2017
Here we describe a novel diabetic murine model utilizing hairless mice for real-time, non-invasive, monitoring of biofilm wound infections of bioluminescent Pseudomonas aeruginosa. This method can be adapted to evaluate infection of other bacterial species and genetically modified microorganisms, including multi-species biofilms, and test the efficacy of antibiofilm strategies.
05:46
A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate
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Cited by 7 •
2017
Here, we present a protocol to perform a semi-high throughput cell migration assay on a 96-well cell culture plate. This protocol is a fast, simple and economical method to create consistent scratch wounds on a cell monolayer.
02:07
Bioluminescence Imaging to Track Bacterial Infection and Wound Healing in a Mouse Model
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2026
Source: Hunt, A. M. A., et al. Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model. J. Vis. Exp.(2017).This video demonstrates the procedure for assessing infection clearance and healing progression in a wounded diabetic mouse using bioluminescent and microscopic images.
FAQs
Chronic hyperglycemia disrupts several processes needed for coordinated repair, including blood vessel function, immune responses, extracellular matrix remodeling, and cell migration. Because these systems operate across successive healing stages, impairment in more than one pathway can prevent efficient progression from hemostasis and inflammation toward tissue regeneration. This multisystem disruption helps explain why repair remains prolonged in diabetes.
Poor oxygen delivery limits the tissue conditions needed for regeneration, while prolonged inflammation prevents the wound from progressing normally toward rebuilding. These problems reinforce the broader effects of diabetes on vascular and immune function. Bioengineering strategies therefore often prioritize improved oxygen-related vascular support and better control of the wound environment rather than addressing cell growth alone.
Engineered scaffolds and hydrogel dressings are designed to modify the local wound environment while tissue repair occurs. Their roles can include supporting tissue organization and maintaining moisture balance, two conditions relevant to regeneration in diabetic wounds. In bioengineering research, these materials provide platforms for developing advanced treatments that respond to impaired repair rather than relying only on conventional wound coverage.
These approaches target different aspects of the impaired healing environment. Controlled drug delivery can regulate therapeutic exposure, while cell- or growth factor-based therapies are designed to support regenerative processes. When combined with engineered materials, they can be incorporated into treatment systems intended to improve angiogenesis, tissue organization, or other repair conditions disrupted by diabetes.
Design begins with the specific barriers that must be addressed, including poor oxygen delivery, persistent inflammation, inadequate moisture balance, limited angiogenesis, and disorganized tissue repair. Researchers can then select or combine scaffolds, hydrogels, delivery systems, cells, or growth factors according to those needs. This process links material design with the biological requirements of regeneration in diabetic tissue.
Bioengineering approaches serve both therapeutic and investigative purposes. Engineered scaffolds, hydrogels, and delivery systems support advanced wound-treatment development, while experimental models help examine how metabolic disease alters regeneration. Studying these changes can connect disrupted vascular, immune, matrix, and cellular behavior with observable repair outcomes, providing a framework for evaluating strategies that aim to restore tissue organization.