Direct Write Assembly
Direct Write Assembly - Related Videos
10:36
Prescribed 3-D Direct Writing of Suspended Micron/Sub-micron Scale Fiber Structures via a Robotic Dispensing System
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Cited by 6 •
2015
Here, we present a protocol to fabricate freely-suspended, micron/sub-micron scale polymer fibers and “web-like” structures generated via automated direct writing procedure by means of a 3-axis dispensing system.
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
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Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
09:34
Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
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Cited by 2 •
2020
A protocol is presented for the synthesis of information-encoded peptoid oligomers and for the sequence-directed self-assembly of these peptoids into molecular ladders using amines and aldehydes as dynamic covalent reactant pairs and Lewis acidic rare-earth metal triflates as multi-role reagents.
08:48
Writing Bragg Gratings in Multicore Fibers
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Cited by 3 •
2016
We describe a technique for inscribing identical fiber Bragg gratings into each core of a multicore fiber. This is achieved by introducing an additional surface into the optical path to mitigate lensing by the curved surface of the fiber cladding.
08:00
Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering
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Cited by 39 •
2011
This article outlines a versatile method to create cell-derived tissue rings by cellular self-assembly. Smooth muscle cells seeded into ring-shaped agarose wells aggregate and contract to form robust three-dimensional (3D) tissues within 7 days. Millimeter-scale tissue rings are conducive to mechanical testing and serve as building blocks for tissue assembly.
FAQs
Computer-controlled paths translate a digital design into specific deposition locations, controlling where material accumulates within each layer. This localized placement helps reproduce patterned geometries and makes structural features intentional rather than arbitrary. In bioengineering, such control is especially valuable for investigating how scaffold architecture, including organized pores, influences cell behavior.
Solidification or crosslinking allows the deposited feedstock to retain the geometry specified by the digital design. Without this structural-retention step, successive layers could not preserve the intended architecture. Depending on the construct, the feedstock may be a polymer, hydrogel, or bioink, making this mechanism relevant to scaffolds, microfluidic features, and cell-containing structures.
Pore organization provides a controllable structural feature for examining relationships between geometry and cell behavior. Direct Write Assembly can place material along designed paths so that pore patterns become part of the construct rather than an incidental result. This capability supports tissue-engineering scaffold development and studies of how biomaterials and architecture affect biological responses.
A typical workflow begins with a computer-based design that specifies the desired geometry and deposition paths. A selected feedstock is then forced through a nozzle and placed layer by layer according to those paths. As the material solidifies or crosslinks, the deposited layers retain the planned architecture, creating a reproducible physical construct from the digital pattern.
The approach can use feedstocks such as polymers, hydrogels, or bioinks. These materials are deposited through a nozzle and must retain the intended arrangement through solidification or crosslinking. This range supports different bioengineering formats, including patterned scaffolds, microfluidic features, and constructs that contain cells within designed three-dimensional architectures.
Researchers may choose this method when they need customized three-dimensional structures with controlled geometry or organized pores. Its digital design-to-placement workflow supports tissue-engineering scaffolds, microfluidic features, and cell-containing constructs. The method is also useful when reproducible fabrication is needed to compare how different structural arrangements or biomaterials influence cell behavior.
The resulting constructs provide defined physical settings for studying the relationship between structure, biomaterials, and cell behavior. Because material placement follows computer-controlled paths, researchers can create customized architectures reproducibly and examine patterned differences across constructs. In bioengineering, this supports investigations of tissue-engineering designs, microfluidic organization, and cell-containing material systems.