Extracellular Fluorescence Measurement
Extracellular Fluorescence Measurement - Related Videos
03:04
Measurement of Extracellular Fluorescent Glucose Analog Depletion: A Surrogate Measurement Technique to Assess Glucose Uptake in Mouse Organs Ex Vivo
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2025
In this video, we demonstrate an ex vivo technique to measure extracellular glucose analog depletion in harvested mouse organs. It involves incubating the mouse tissue in a solution containing fluorescently-labeled deoxyglucose, which is taken up by the cells. The glucose analog uptake can be indirectly determined by measuring the fluorescence of the incubated medium.
05:52
In Vitro Glucose Depletion Assay: A Fluorescence-Based Method to Quantify Cellular Glucose Uptake via Extracellular Glucose Depletion Measurement
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2025
This video demonstrates an in vitro assay to indirectly measure intracellular glucose uptake. The assay uses a fluorescently-labeled glucose analog to establish a correlation between extracellular glucose depletion and intracellular glucose uptake.
06:45
A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
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Cited by 8 •
2016
Neutrophil extracellular traps (NETs) are networks of DNA, histones and neutrophil proteins. Although a component of the innate immune response, NETs are implicated in autoimmunity and thrombosis. This protocol describes a simple method for canine neutrophil isolation and quantification of NETs using a microplate fluorescence assay.
06:47
Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate
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Cited by 103 •
2015
Glycolysis is a defining metabolic marker in multiple biological systems. Monitoring glycolysis by measuring the extracellular flux of H+ is common, but requires correction to be quantitative and unambiguous. Here, we demonstrate how to gather and correct extracellular flux data to distinguish between respiratory and glycolytic sources of extracellular acidification.
12:33
High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
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Cited by 485 •
2013
To measure potential rates of soil extracellular enzyme activities, synthetic substrates that are bound to a fluorescent dye are added to soil samples. Enzyme activity is measured as the fluorescent dye is released from the substrate by an enzyme-catalyzed reaction, where higher fluorescence indicates more substrate degradation.
FAQs
The fluorophore provides the measurement’s optical selectivity: it absorbs light at a chosen excitation wavelength and then emits light at a longer wavelength. The detector records that emitted signal from the extracellular medium. This wavelength-dependent sequence allows researchers to monitor and quantify changes in fluorescence while distinguishing the measured signal from the light used to stimulate the fluorophore.
An extracellular signal can represent several biological events, so interpretation depends on the labeled material and experimental question. Changes in fluorescence may be associated with secretion, molecular release, altered membrane permeability, extracellular enzymatic activity, or changes in a labeled analyte. The optical result therefore becomes meaningful when connected to the specific extracellular process under investigation.
Because the measurement samples the surroundings without disrupting cells, researchers can follow cellular behavior while the experiment continues. This is useful for observing secretion, transport, signaling, or responses to experimental conditions over time. Preserving the cells during measurement helps maintain the biological context that the experiment is intended to examine.
A basic workflow begins by selecting a fluorophore associated with the extracellular target or event, exposing the sample to its excitation wavelength, and recording emitted light from the surrounding medium. Researchers then quantify or compare the detected fluorescence under the conditions of interest. This sequence supports observation of extracellular responses as they change during an experiment.
The extracellular medium is the compartment in which signals linked to outside-cell processes are detected. Fluorescence changes there can provide evidence of released or secreted material, extracellular enzymatic activity, membrane permeability changes, or altered labeled analytes. Examining this compartment allows researchers to assess aspects of cell function without directly disrupting the cells being studied.
Researchers can apply extracellular fluorescence measurements when they need to track cell signaling, transport, secretion, molecular release, or responses to experimental conditions without interrupting the sample. The approach is also useful for monitoring labeled analytes and extracellular enzymatic activity. Its real-time capability makes it relevant to experiments focused on changing cellular behavior and surrounding environments.