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High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
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High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

DOI:
10.3791/50961-v

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12:33 min

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November 15, 2013

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, , , , ,
1Natural Resource Ecology Laboratory,Colorado State University, 2Biosciences Division,Oak Ridge National Laboratory, 3Department of Bioengineering,University of Colorado

Chapters

  • 00:05Title
  • 02:18Assay Set-up
  • 04:00Incubation of Assay Plates
  • 04:56Fluorescent Measurements on a Microplate Fluorometer
  • 05:43Data Analysis
  • 07:03Results: Analysis of Extracellular Enzyme Activity (EEA)
  • 11:05Conclusion

Summary

Automatic Translation

Automatic Translation

To measure potential rates of soil extracellular enzyme activities, synthetic substrates that are bound to a fluorescent dye are added to soil samples. Enzyme activity is measured as the fluorescent dye is released from the substrate by an enzyme-catalyzed reaction, where higher fluorescence indicates more substrate degradation.

Tags

Keywords: Soil Extracellular Enzyme ActivitiesFluorometric MeasurementSoil Microbial Enzyme ActivityFluorescence Enzyme AssaySoil SlurriesFluorescently Labeled SubstratesPotential Enzyme ActivitySoil Ecosystem Functional DynamicsEnzyme Kinetics

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