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JoVE Journal
Environment

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High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
 

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Article doi: 10.3791/50961
November 15th, 2013

Summary November 15th, 2013

To measure potential rates of soil extracellular enzyme activities, synthetic substrates that are bound to a fluorescent dye are added to soil samples. Enzyme activity is measured as the fluorescent dye is released from the substrate by an enzyme-catalyzed reaction, where higher fluorescence indicates more substrate degradation.

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