Krebs Ringer Hepes
Krebs Ringer Hepes - Related Videos
08:58
En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
0 Views •
Cited by 10 •
2016
In this article we describe an adapted relatively easy method using the fluorescence dye diaminofluorescein-2 diacetate (DAF-2DA) and dihydroethidium (DHE) for en face simultaneous detection and visualization of intracellular nitric oxide (NO) and superoxide anion (O2.−) respectively, in freshly isolated intact aortas of an obesity mouse model.
04:04
Radiolabeled Amino Acid Uptake Assay to Quantify Cellular Uptake of Amino Acids
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2025
This video demonstrates an in vitro technique for estimating amino acid uptake by bone marrow-derived stromal cells. Na+-dependent amino acid transporters mediate the uptake of radiolabeled amino acids inside the cells, and the cellular uptake is estimated by measuring the radioactivity.
07:55
A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device
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Cited by 5 •
2010
A microfluidic islet perifusion device was developed for the assessment of dynamic insulin secretion of multiple islets and simultaneous fluorescence imaging of calcium influx and mitochondrial potential changes.
08:42
Myo-mechanical Analysis of Isolated Skeletal Muscle
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Cited by 25 •
2011
To assess the in vivo effects of therapeutic interventions for muscle disease, methods are needed to quantitate force generation and fatigability in treated muscle. We detail an approach to evaluating myo-mechanical properties in explanted mouse hindlimb muscle. This analysis provides a robust approach to quantitating the effects of genetic modification on muscle function, as well as comparison of therapies in mouse models of muscle disease.
13:47
TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
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Cited by 8 •
2015
This paper provides a method for investigating neurotransmitter vesicle dynamics in neuroblastoma cells, using a synaptobrevin2-pHluorin construct and Total Internal Reflection Fluorescence Microscopy. The strategy developed for image processing and data analysis is also reported.
FAQs
The solution supplies balanced concentrations of inorganic ions that help preserve osmotic stability around isolated cells, tissues, and organs. This stable ionic environment supports cellular function during handling or incubation, reducing the likelihood that responses arise from abrupt changes in extracellular conditions. Consequently, observed secretion, transport, or metabolic activity can be interpreted under more controlled experimental conditions.
HEPES provides buffering capacity that helps maintain a near-constant pH when an experiment does not use a carbon dioxide-controlled atmosphere. This is important because isolated biological material can respond to changing acidity independently of the experimental treatment. By limiting pH variation, the buffer helps preserve the intended extracellular conditions during laboratory procedures and measurements.
Ionic balance and pH jointly influence the extracellular environment experienced by isolated biological material. If either condition changes substantially, cellular responses may reflect environmental stress rather than the process under study. Krebs Ringer HEPES therefore improves experimental consistency by stabilizing two major variables at once, helping investigators distinguish genuine biological effects from artifacts caused by solution conditions.
The controlled environment supports assays that examine secretion, transport, or metabolism. Because the solution helps limit changes in pH and ionic conditions, measurements are less confounded by fluctuations in the surrounding medium. This allows researchers to focus on how the isolated cells, tissues, or organs respond during the assay rather than on secondary effects of an unstable extracellular environment.
During tissue preparation or cell incubation, the solution provides the surrounding medium under controlled laboratory conditions. Its balanced inorganic ions help maintain osmotic stability, while HEPES helps limit pH variation, particularly when no carbon dioxide-controlled atmosphere is available. These properties support more consistent handling and incubation before researchers assess cellular or tissue responses.
In organ perfusion, Krebs Ringer HEPES serves as the controlled solution surrounding or passing through the isolated organ during the experiment. Maintaining balanced ionic conditions and buffering capacity helps preserve a consistent extracellular environment throughout the procedure. This is useful when investigators measure organ-level secretion, transport, or metabolic responses and need to separate those responses from solution-induced changes.
Researchers may select it when an assay requires isolated cells, tissues, or organs to remain under stable extracellular conditions, especially outside a carbon dioxide-controlled atmosphere. It is relevant to experiments involving tissue preparation, incubation, perfusion, secretion, transport, or metabolism. The choice supports reproducibility by reducing variation caused by changing pH or ionic conditions during the measurement.
The solution creates a more consistent background against which biological responses can be measured. Stabilizing osmotic conditions, inorganic ion concentrations, and pH reduces the chance that changing extracellular conditions will mimic or obscure the effect being tested. As a result, differences observed between experimental conditions can be attributed more confidently to the biological process under investigation.