Acidosis
Acidosis - Related Videos
01:24
Diagnosing Acidosis and Alkalosis
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2024
Diagnosing acid-base imbalances involves systematically analyzing arterial blood samples, focusing on three key measurements: pH, bicarbonate (HCO3−) concentration, and carbon dioxide partial pressure (PCO2). This analysis follows a four-step process that helps identify the imbalance's underlying cause and nature. First, the pH level is assessed to determine whether the blood pH is normal (7.35–7.45), low (acidosis), or high (alkalosis). Next, the PCO2 and HCO3− values are examined to...
08:56
Modeling Mitochondrial Disease Using Brain Organoids: A Focus on Mitochondrial Encephalomyopathy, Lactic Acidosis, and Stroke-like Episodes
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Cited by 1 •
2025
Brain organoids serve as a valuable model for Mitochondrial Encephalomyopathy, Lactic Acidosis, and Stroke-like episodes (MELAS) studies, offering insights into their underlying pathophysiology and providing a platform for drug screening. Organoids derived from cell lines with varying levels of heteroplasmy of disease-causing genes exhibit significant phenotypic differences.
10:13
Experimental Models to Study the Neuroprotection of Acidic Postconditioning Against Cerebral Ischemia
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Cited by 3 •
2017
Acidic postconditioning protects against cerebral ischemia. Here we present two models to execute APC. They are achieved respectively by transferring corticostriatal slices to acidic buffer after oxygen-glucose deprivation in vitro and by inhaling 20% CO2 after middle cerebral artery occlusion in vivo.
02:29
Assessing the Neuroprotective Effects of Acidic Postconditioning in a Mouse Model of Cerebral Ischemia
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2025
Source: Zheng, Y., et al. Experimental Models to Study the Neuroprotection of Acidic Postconditioning Against Cerebral Ischemia. J. Vis. Exp. (2017)This video demonstrates the application of acidic postconditioning (APC) to evaluate its neuroprotective effects against ischemic injury in a mouse model of middle cerebral artery occlusion (MCAO). It outlines the steps involved in reperfusion in the MCAO model and the application of APC during reperfusion to mitigate excitotoxicity, oxidative...
10:15
Steps for the Autologous Ex vivo Perfused Porcine Liver-kidney Experiment
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Cited by 2 •
2013
Study of the animal organ physiology can be achieved by performing an experimental ex vivo perfusion system. The addition of a porcine kidney, as a homeostatic organ to our previously developed ex vivo liver perfusion model can be a principal step to achieve a better physiological environment.
FAQs
Chemical buffers, the lungs, and kidneys form a coordinated system that limits changes in body-fluid acidity. When ventilation is impaired, carbon dioxide retention can increase the acid burden. Metabolic disturbances may instead involve bicarbonate loss or hydrogen ion accumulation. Examining which component is affected helps explain how acid–base regulation becomes disrupted.
Respiratory acidosis is linked primarily to increased carbon dioxide retention caused by impaired ventilation. Metabolic acidosis arises from disturbances such as bicarbonate loss or increased hydrogen ion accumulation that are not primarily described as a ventilation problem. Distinguishing these pathways allows researchers to connect an altered pH state with its underlying physiological source.
Acidity influences the chemical conditions in which cellular processes operate. When body fluids become excessively acidic, enzyme activity, membrane transport, and energy metabolism may be disrupted, which can impair cellular and organ function. This connection makes acidosis useful for studying how stable acid–base conditions support normal biological activity.
Cells function within changing internal and environmental conditions, so shifts in acidity provide a way to examine physiological adaptation. Studying acidosis reveals how altered acid–base conditions challenge enzyme activity, transport across membranes, and energy metabolism. These effects help connect molecular changes with broader cellular and organ responses.
Researchers interpret physiological measurements by relating them to the possible source of acid–base disruption. Evidence of carbon dioxide retention points toward impaired ventilation, while bicarbonate loss or other metabolic disturbances indicates a different pathway. This approach links measured physiological changes with the mechanisms affecting body-fluid acidity and organ function.
Acidosis is especially relevant when investigating respiratory and metabolic disease, because its mechanisms connect impaired ventilation or metabolic disturbance with altered cellular conditions. It also supports analysis of physiological measurements and cellular adaptation. In biology, these applications help explain how disruptions in acid–base regulation influence tissues, organs, and energy-dependent processes.