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Experimental Models to Study the Neuroprotection of Acidic Postconditioning Against Cerebral Ischemia
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JoVE Journal Neuroscience
Experimental Models to Study the Neuroprotection of Acidic Postconditioning Against Cerebral Ischemia

Experimental Models to Study the Neuroprotection of Acidic Postconditioning Against Cerebral Ischemia

DOI:
10.3791/55931-v

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10:13 min

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July 31, 2017

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, , , , , ,
1Institute of Pharmacology & Toxicology, College of Pharmaceutical Sciences, Department of Pharmacology, Key Laboratory of Medical Neurobiology of The Ministry of Health of China, Zhejiang Province Key Laboratory of Neurobiology,Zhejiang University

Chapters

  • 00:05Title
  • 00:57Cortical Slice Preparation
  • 02:43Oxygen-glucose Deprivation and Acidic Postconditioning
  • 04:03Determination of Slice Viability
  • 05:04Middle Cerebral Artery Occlusion
  • 07:22Reperfusion and Acidic Postconditioning
  • 08:16Results: Acidic Preconditioning Protects Against Reperfusion Injury
  • 09:32Conclusion

Summary

Automatic Translation

Automatic Translation

Acidic postconditioning protects against cerebral ischemia. Here we present two models to execute APC. They are achieved respectively by transferring corticostriatal slices to acidic buffer after oxygen-glucose deprivation in vitro and by inhaling 20% CO2 after middle cerebral artery occlusion in vivo.

Tags

NeuroprotectionAcidic PostconditioningCerebral IschemiaCortico Striatal Slice ModelMiddle Cerebral Artery Occlusion ModelIschemic Brain InjuryStroke TherapyAcidosis PostconditioningBrain Slice PreparationGlucose-free ACSFAcidic ACSFSynaptic Function Recovery

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