Adult Heart Mounting
Adult Heart Mounting - Related Videos
06:51
Recovery of Adult Zebrafish Hearts for High-throughput Applications
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Cited by 16 •
2014
Use of zebrafish for cardiovascular research is expanding towards research on adult hearts. For these applications, quick and simple isolation of cardiac tissues is key to avoid post-mortem changes and to obtain an adequate number of samples. Here, we describe a fast and reproducible method for dissecting adult zebrafish hearts.
05:52
Preparation of Drosophila Heart: A Technique to Study Heart Physiology in the Adult Fly
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2023
This video describes the fly's adult heart, or otherwise called the Drosophila dorsal vessel. The featured protocol demonstrates a dissection procedure for live heart preparations.
12:02
Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea
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Cited by 85 •
2016
We present a method to isolate the adult organ of Corti as three intact cochlear turns (apex, middle, and base). We also demonstrate a procedure for immunostaining with fluorescently tagged antibodies. Together these techniques allow the study of hair cells, supporting cells, and other cell types found in the cochlea.
02:56
Intrathoracic Injection: Compound Delivery to the Adult Zebrafish Heart
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2023
This video describes the technique of delivering compounds into the heart of adult zebrafish by using intrathoracic injection.
11:44
Primary Culture of Adult Rat Heart Myocytes
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Cited by 26 •
2009
In this paper, we described a typical way to isolate and culture adult rat heart myocytes. Collagenase and protease are used to digest and isolate single myocytes. Myocytes cultured follow this protocol meet most experiment requirements.
FAQs
A defined orientation determines which anatomical surfaces and internal structures can be accessed consistently. Positioning the excised heart in relation to the chambers, valves, vessels, or myocardial surfaces helps researchers compare specimens and align observations across experiments. This consistency is especially important when structural features must be connected with microscopy, histology, or other downstream measurements.
Tissue support helps preserve anatomical relationships while researchers expose selected cardiac regions. Without adequate support, handling may produce excessive deformation that complicates interpretation of chamber shape, valve position, vessel arrangement, or myocardial organization. Maintaining the specimen’s structure therefore improves the reliability of later imaging, sectioning, immunostaining, and structural assessment.
The preparation can facilitate examination of chambers, valves, vessels, and myocardial surfaces, depending on how the heart is positioned. Exposing these features while retaining their relationships allows structural observations to remain connected to the intact anatomical arrangement of the excised organ. That connection supports analysis of tissue organization rather than viewing isolated features without spatial context.
The workflow begins with an excised adult heart, followed by placement in a selected orientation that supports the intended examination. The tissue is then secured so relevant anatomical regions remain accessible and relationships are preserved during handling. Once mounted, the preparation can proceed to dissection, imaging, histological sectioning, immunostaining, or structural analysis.
Researchers use this preparation when cardiac anatomy must remain stable and consistently presented during microscopy, imaging, histological sectioning, or immunostaining. It is useful for examining myocardial surfaces and internal structures while limiting deformation that could affect interpretation. The resulting consistency helps compare specimens and relate visible morphology to tissue-level organization.
In cardiovascular biology, a well-prepared mount helps connect cardiac anatomy with tissue organization, disease-related remodeling, and experimental measurements. Researchers can examine how structural features correspond to changes in the myocardium or other cardiac regions, while a consistent orientation supports comparison between preparations. This makes mounting a foundation for studying adult heart structure and function.