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Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea
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Neuroscience
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JoVE Journal Neuroscience
Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea

Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea

DOI:
10.3791/53561-v

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12:02 min

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January 01, 2016

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1Department of Surgery, Division of Otolaryngology,Southern Illinois University, School of Medicine, 2Department of Pharmacology,Southern Illinois University, School of Medicine

Chapters

  • 00:05Title
  • 00:59Preparation of Temporal Bones
  • 02:14Whole Mount Dissection of the Cochlea
  • 05:41Immunostaining with Fluorescently Tagged Antibodies
  • 07:30Mounting Stained Cochlear Turns
  • 08:59Results: Examples of Optimal and Suboptimal Dissection and Immunostaining
  • 10:54Conclusion

Summary

Automatic Translation

Automatic Translation

We present a method to isolate the adult organ of Corti as three intact cochlear turns (apex, middle, and base). We also demonstrate a procedure for immunostaining with fluorescently tagged antibodies. Together these techniques allow the study of hair cells, supporting cells, and other cell types found in the cochlea.

Tags

Keywords: CochleaWhole Mount DissectionImmunofluorescenceOrgan Of CortiTemporal BoneOtic CapsuleSpiral LigamentDecalcificationForcepsVannas-Tubingen ScissorsConfocal MicroscopyZ-axis Imaging

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