Journal
/
/
Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface
DOI:

10:10 min

February 23, 2020

, , , , , , , , , ,

Chapters

  • 00:05Introduction
  • 00:31Seeding of Cells onto Microporous Membranes in Cell Culture Inserts
  • 01:58Pressing of Test Substances
  • 03:07Leakage Test of the Radial Flow System
  • 03:51Aerosol Generation
  • 05:09Exposure Experiments
  • 07:30Cell Viability
  • 08:27Results: Viability Data
  • 09:11Conclusion

Summary

Automatic Translation

We present a robust, transferable and predictive in vitro exposure system for the screening and monitoring of airborne particles concerning their acute pulmonary cytotoxicity by exposing cultivated human lung cells at the air-liquid interface (ALI).

Related Videos

Read Article