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An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing
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Biology
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JoVE Journal Biology
An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

DOI:
10.3791/61210-v

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09:29 min

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May 13, 2020

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, , , , , , , , ,
1National Institute for Public Health and the Environment (RIVM), 2Institute for Risk Assessment Sciences (IRAS)

Chapters

  • 00:04Introduction
  • 00:54Seeding Calu-3 Cells onto Culture Inserts
  • 03:09Preparing the Exposure Setup
  • 05:14Handling the AES During an Exposure
  • 07:02Results: Cell Model Selection
  • 08:53Conclusion

Summary

Automatic Translation

Automatic Translation

Described is the cell culture and exposure method of an in vitro bronchial model for realistic, repeated inhalation exposure to particles for toxicity testing.

Tags

Keywords: Air-liquid InterfaceBronchial Epithelial CellsInhalation ExposureToxicity TestingTransepithelial Electrical ResistanceCell CultureAir-liquid Interface CultureAirborne Particles

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