Arabidopsis Cotyledons
Arabidopsis Cotyledons - Related Videos
12:01
Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination
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Cited by 26 •
2012
We describe a protocol using chamber slides and media to immobilize plant cotyledons for confocal imaging of the epidermis over several days of development, documenting stomatal differentiation. Fluorophore-tagged proteins can be tracked dynamically by expression and subcellular localization, increasing understanding of their possible roles during cell division and cell-type differentiation.
10:24
A Method for Characterizing Embryogenesis in Arabidopsis
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Cited by 11 •
2017
This protocol outlines a method for observing embryogenesis in Arabidopsis via ovule clearance followed by the inspection of embryo pattern formation under a microscope.
09:23
Lateral Root Inducible System in Arabidopsis and Maize
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Cited by 8 •
2016
The Lateral Root Inducible System (LRIS) allows for synchronous induction of lateral roots and is presented for Arabidopsis thaliana and maize.
08:13
Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
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Cited by 143 •
2017
The objective of this study was to determine the effects of bleach and chlorine gas sterilization on seed germination of a range of Arabidopsis genotypes grown on sterile media. Optimized sterilization protocols have been developed to prevent the growth of microbial contaminants while providing satisfactory seed survival.
03:03
Inoculating a Bacterial Pathogen into Arabidopsis Leaves via Vacuum Infiltration
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2026
Source: Rufián, J. S., et.al. Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a vacuum infiltration method to introduce a bacterial pathogen into Arabidopsis plant leaves. The approach uses a prepared bacterial suspension and vacuum pulses to enable uniform entry through stomata, resulting in controlled leaf infection.
FAQs
Light acts as both an environmental cue and a developmental signal. In response, cotyledons expand and develop chloroplasts, the organelles associated with photosynthesis. This shift allows the seedling to begin producing energy through photosynthesis while establishing its first true leaves, making cotyledon development a visible readout of light-regulated seedling establishment.
Cotyledon growth responds to environmental and hormonal signals, so changes in their size or development can reveal how plants coordinate external conditions with internal regulation. Examining these responses helps researchers connect signaling processes to seedling establishment, cellular differentiation, and the developmental changes that occur before the first true leaves become established.
Cotyledons provide a readily observed system for studying photomorphogenesis, the developmental response to light. Their emergence, expansion, and chloroplast development link light perception with metabolism and visible growth. Because these changes occur during early seedling establishment, they help clarify how plants translate environmental information into coordinated developmental transitions.
Cotyledons represent an early developmental stage that precedes the establishment of the first true leaves. This timing lets researchers use cotyledon growth to examine how a seedling progresses from emergence and initial expansion toward later leaf development. Comparing these stages can therefore distinguish early establishment processes from subsequent developmental transitions.
A study can follow cotyledons across the sequence of post-germination events: emergence from the seed, expansion, light-associated chloroplast development, and establishment of the first true leaves. These visible milestones provide a practical framework for examining how environmental or hormonal conditions influence seedling establishment and the timing of developmental change.
This system is particularly useful for questions involving seedling establishment, photomorphogenesis, leaf development, and cellular differentiation. Researchers can examine how cotyledon growth changes as light, environmental conditions, or hormonal signals affect the seedling. The resulting observations help connect early visible development with underlying physiological and cellular processes.
Their accessible early growth stage brings together several major plant processes: light perception, metabolism, photosynthetic development, and developmental transitions. Studying these processes in one visible structure helps researchers investigate how plants coordinate environmental information with growth. Findings from this model can therefore support broader understanding of early plant development and cellular specialization.