Long-term, High-resolution Confocal Time Lapse Imaging of <em>Arabidopsis Cotyledon</em> Epidermis during Germination

Kylee M. Peterson; Keiko U. Torii

doi:10.3791/4426

Journal

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Biology

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Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination

Long-term, High-resolution Confocal Time Lapse Imaging of <em>Arabidopsis Cotyledon</em> Epidermis during Germination

JoVE Journal
Biology

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JoVE Journal Biology

Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination

Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination

13,734 Views

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12:01 min

•

December 31, 2012

DOI:

10.3791/4426-v

DOI:

10.3791/4426-v

•

12:01 min

December 31, 2012

•

9 Views

Kylee M. Peterson¹, Keiko U. Torii^1,2,3

¹Department of Biology,University of Washington, ²Howard Hughes Medical Institute,University of Washington, ³PRESTO,Japan Science and Technology Agency

Summary

We describe a protocol using chamber slides and media to immobilize plant cotyledons for confocal imaging of the epidermis over several days of development, documenting stomatal differentiation. Fluorophore-tagged proteins can be tracked dynamically by expression and subcellular localization, increasing understanding of their possible roles during cell division and cell-type differentiation.