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Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination
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Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination

Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination

DOI:
10.3791/4426-v

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12:01 min

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December 31, 2012

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,
1Department of Biology,University of Washington, 2Howard Hughes Medical Institute,University of Washington, 3PRESTO,Japan Science and Technology Agency

Chapters

  • 00:05Title
  • 01:00Seed Sterilization
  • 01:57Preparation of Chamber Slide
  • 03:11Seed Dissection
  • 04:47Mounting Cotyledons in the Chamber Slide
  • 05:40Time Lapse Imaging
  • 09:29Results: Changes in Localization of POLAR Protein during Proliferation Stage of Stomatal-lineage Cells in the Cotyledon Epidermis
  • 11:40Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol using chamber slides and media to immobilize plant cotyledons for confocal imaging of the epidermis over several days of development, documenting stomatal differentiation. Fluorophore-tagged proteins can be tracked dynamically by expression and subcellular localization, increasing understanding of their possible roles during cell division and cell-type differentiation.

Tags

Confocal Time Lapse ImagingArabidopsis CotyledonCellular BehaviorIn Vivo DynamicsPlant DevelopmentLong-term Live ImagingCell DivisionDifferentiation EventsArabidopsis EpidermisSignalingCell FateStomataAsymmetric DivisionCell Differentiation Steps

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