Arabidopsis Wounding

Arabidopsis wounding is the study of how Arabidopsis thaliana responds to mechanical injury, providing a tractable model for plant stress signaling, defense, and tissue repair. When leaves or stems are damaged, disrupted cells generate calcium and reactive oxygen signals that spread locally and systemically, activate jasmonate signaling, and reprogram gene expression near and beyond the wound. Researchers use controlled damage, imaging, transcript analysis, and mutant plants to identify the signaling pathways that coordinate defense compounds, wound closure, and growth responses. These experiments clarify how plants communicate injury without a nervous system and connect molecular signaling with crop resilience and regenerative biology.

Arabidopsis Wounding - Related Videos

Research

JoVE Journal - Medicine

Demonstration of the Rat Ischemic Skin Wound Model

0 Views •

Cited by 32 •

2015

The rat, due to its size, availability, and rather docile behavior, has been utilized as a research model for many years. The goal of this protocol is to utilize the rat as an ischemic skin wound healing model to provide valuable insight into the pathophysiology of chronic wounds.

A Method for Characterizing Embryogenesis in Arabidopsis

0 Views •

Cited by 11 •

2017

This protocol outlines a method for observing embryogenesis in Arabidopsis via ovule clearance followed by the inspection of embryo pattern formation under a microscope.

Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis

0 Views •

Cited by 7 •

2021

Extracellular glutamate-triggered systemic calcium signaling is critical for the induction of plant defense responses to mechanical wounding and herbivore attack in plants. This article describes a method to visualize the spatial and temporal dynamics of both these factors using Arabidopsis thaliana plants expressing calcium- and glutamate-sensitive fluorescent biosensors.

An Assay to Detect Oxidative Burst in Arabidopsis Leaf Discs upon Immune Elicitation

0 Views •

2025

This video demonstrates an assay to measure oxidative burst in Arabidopsis leaves upon immune elicitation. Leaf discs, cut from plant leaves, are incubated with bacterial peptides functioning as microbe-associated molecular patterns or MAMPs. Upon detection of the MAMPs, plant cells induce the production of extracellular ROS, which is detected using a chemiluminescence assay.

Collecting And Measuring Wound Exudate Biochemical Mediators In Surgical Wounds

0 Views •

Cited by 6 •

2012

This article provides a detailed and visual description of a methodology for collecting and measuring biochemical inflammatory and nociceptive mediators at the surgical wound site following cesarean delivery. This human bioassay has been used to determine correlations between wound and serum cytokine concentrations and drug-mediated changes in wound cytokines, chemokines and neuropetides.

View All Results

FAQs

Related Topics