Calcium Flux Assessment
Calcium Flux Assessment - Related Videos
12:10
Measuring Fast Calcium Fluxes in Cardiomyocytes
0 Views •
Cited by 3 •
2011
We present a method to isolate rapid (microsecond) calcium events from slower fluxes in living cells using laser scanning confocal microscopy. The method measures fluorescence intensity fluctuations of calcium indicators by recording line scans of several hundred pixels in a cell. Histogram analysis allows us to isolate the time scales of different calcium fluxes.
03:54
Monitoring B Cell Activation with Antigenic Liposomes using a Calcium-Flux Assay
0 Views •
2025
This video showcases the assessment of B cell activation through the utilization of antigenic liposomes. B cells treated with calcium-sensitive Indo-1 dye are distinguished using flow cytometry, and their violet-to-blue fluorescence ratio is analyzed. As the cells encounter antigenic liposomes, it triggers B cell activation through calcium influx, progressively enhancing the violet-to-blue fluorescence signal ratio over time.
08:14
Assessing T-cell Receptor-Induced Calcium Influx Using a Calcium Indicator Dye
0 Views •
2025
This video analyzes T-cell receptor-induced calcium influx in T-cells. Anti-CD3 antibodies activate the T-cell signaling pathways, leading to an increase in intracellular calcium ions that bind with Indo-1 dye. In flow cytometry, a spectrum shift from the calcium-free state to the calcium-bound state correlates with T-cell receptor-induced calcium influx.
05:04
In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter
0 Views •
Cited by 4 •
2022
We describe here, the establishment and application of an Tg(Myh6-cre)1Jmk/J /Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J (referred to as αMHC-Cre/Rosa26A-Flox-Stop-Flox-GCaMP3 below) mouse reporter line for cardiac reprogramming assessment. Neonatal cardiac fibroblasts (NCFs) isolated from the mouse strain are converted into induced cardiomyocytes (iCMs), allowing for convenient and efficient evaluation of reprogramming efficiency and functional maturation of iCMs via calcium (Ca2+) flux.
11:22
Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers
0 Views •
Cited by 15 •
2014
Described here is a method to directly measure calcium sparks, the elementary units of Ca2+ release from sarcoplasmic reticulum in intact skeletal muscle fibers. This method utilizes osmotic-stress-mediated triggering of Ca2+ release from ryanodine receptor in isolated muscle fibers. The dynamics and homeostatic capacity of intracellular Ca2+ signaling can be employed to assess muscle function in health and disease.
FAQs
Calcium-sensitive indicators respond when they bind calcium ions, producing a signal that changes with the calcium state being monitored. The resulting trace can be examined for response timing and magnitude, allowing researchers to connect a stimulus with downstream cellular activity. This readout is especially useful when signaling events are too rapid or transient to assess from endpoint observations alone.
These processes represent distinct sources of increased intracellular calcium. Entry occurs when calcium crosses the plasma membrane, whereas release mobilizes calcium already contained within the cell. Calcium flux assessment can help compare their timing and relative contribution to a response, which is relevant for interpreting signaling pathways involved in neuronal communication, contraction, secretion, and gene regulation.
Response timing indicates how quickly a cell reacts and how long the signal persists, while magnitude reflects the relative size of the calcium change detected by the indicator. Comparing these features across conditions can reveal altered signaling behavior rather than merely showing whether a response occurred. Such comparisons help distinguish normal cellular activity from dysfunctional regulation.
A typical assessment exposes cells or tissues to a relevant stimulus while monitoring the signal generated by a calcium-sensitive indicator. Researchers then compare the observed response with a reference condition, focusing on when the signal begins, how large it becomes, and how it changes over time. This workflow links an external challenge to calcium-dependent cellular activity.
The approach supports studies of calcium-dependent events across several biological systems. In neurons, it can characterize signaling associated with communication; in muscle, it can relate calcium changes to contraction. It also helps examine secretion, gene regulation, and other cellular pathways in which calcium acts as an important activity-linked signal.
Researchers can compare calcium responses before and after exposing cells or tissues to a candidate compound. Changes in response timing, magnitude, or overall behavior may indicate that the compound affects a calcium-dependent pathway. This application helps characterize whether signaling is altered under treatment and can support comparisons between normal and dysfunctional cellular responses.