Candida Glabrata
Candida Glabrata - Related Videos
13:47
Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages
0 Views •
Cited by 9 •
2013
The current article outlines a protocol to establish an in vitro cell culture model system to study the interaction of a facultative intracellular human fungal pathogen Candida glabrata with human macrophages which will be a useful tool to advance our knowledge of fungal virulence mechanisms.
08:45
Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
0 Views •
Cited by 22 •
2017
This study implemented whole genome sequencing for analysis of mutations in genes conferring antifungal drug resistance in Candida glabrata. C. glabrata isolates resistant to echinocandins, azoles and 5-flucytosine, were sequenced to illustrate the methodology. Susceptibility profiles of the isolates correlated with presence or absence of specific mutation patterns in genes.
09:27
Generation of Fluorescent Protein Fusions in Candida Species
0 Views •
Cited by 6 •
2017
PCR-mediated gene modification can be used to generate fluorescent protein fusions in Candida species, which facilitates visualization and quantitation of yeast cells and proteins. Herein, we present a strategy for constructing a fluorescent protein fusion (Eno1-FP) in Candida parapsilosis.
07:57
Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis
0 Views •
Cited by 83 •
2011
Key techniques to be used in the evaluation of Candida vaginitis in an experimental animal model are described. The methods will allow rapid collection of vaginal specimens and lymphocytes from draining lumbar lymph nodes. These techniques could give rise to mouse models of other diseases in the female lower genital tract.
03:50
Candida albicans Biofilm Formation in an In Vivo Mouse Model
0 Views •
2025
This video demonstrates the development of an in vivo mouse model to study the biofilm formation of Candida albicans in subcutaneous tissue. The experiment involves implanting Candida-infected devices in the animal's back region for biofilm development.
FAQs
Adhesion helps Candida glabrata remain attached to mucosal surfaces rather than being readily removed. This persistence creates an opportunity for the yeast to maintain contact with host tissue and establish infection when protective barriers or immune defenses are weakened. In biological studies, adhesion is therefore an important process for explaining how colonization can progress toward disease.
Two important responses are increased drug-efflux activity and remodeling of the cell wall. Efflux can lower the amount of antifungal compound retained by the cell, while cell-wall remodeling changes a structure targeted or affected during treatment. Together, these adaptive responses can reduce drug susceptibility and complicate treatment of infections caused by this yeast.
Mutations in FKS genes can further promote resistance to echinocandins, an antifungal treatment class. This genetic change is distinct from broader adaptive responses such as increased drug efflux or cell-wall remodeling because it provides a mutation-associated route to reduced susceptibility. Detecting this possibility is important when interpreting antifungal susceptibility and considering treatment effectiveness.
Species identification establishes which Candida organism is present, while antifungal susceptibility testing evaluates how it responds to available treatment. Using both types of information supports therapy decisions more effectively than relying on identification alone. For Candida glabrata, this pairing is especially relevant because adaptive responses and FKS mutations can reduce susceptibility to antifungal treatment.
Research on Candida glabrata contributes to infection control by clarifying how the yeast persists, acquires reduced drug susceptibility, and becomes clinically important when host defenses or protective barriers are compromised. These findings help frame resistance as both a biological and clinical concern. They also support efforts to recognize organisms that may complicate management of serious infections.
Candida glabrata provides a model for investigating how fungal cells respond to treatment pressure through drug-efflux activity, cell-wall remodeling, and resistance-associated mutations. Understanding these mechanisms can identify biological processes that require closer evaluation during antimicrobial development. The subject also connects laboratory studies of fungal biology with the practical need for therapies that remain effective despite acquired resistance.