Cotton-tipped Applicator
Cotton-tipped Applicator - Related Videos
05:40
Fabricating Cotton Analytical Devices
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2016
To investigate simple fabrication approaches for multiple assay needs, we created a fluid-absorbing channel system made of cotton material. This device was used to establish a multiple detection platform, and solve contamination issues that commonly affect lateral flow-based biomedical devices, for clinical urinalysis of nitrite, total protein, and urobilinogen.
10:18
Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
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Cited by 159 •
2011
We present the detailed protocol for Agrobacterium-mediated virus-induced gene silencing (VIGS) assay in cotton. The tobacco rattle virus (TRV)-derived VIGS vectors were deployed to induce RNA silencing of cotton GrCLA1, Cloroplastos alterados 1 gene. The albino phenotype caused by silencing GrCLA1 was observed at the seedling stage within 2 weeks after inoculation.
06:28
Screening Cotton Genotypes for Reniform Nematode Resistance
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2019
Here, a protocol is presented for the rapid non-destructive screening of cotton genotypes for reniform nematode resistance. The protocol involves visually examining the roots of nematode-infected cotton seedlings to determine infection response. The vegetative shoot from each plant is then propagated to recover plants for seed production.
08:11
Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction
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Cited by 7 •
2017
Methods for a wide-scale central nervous system gene delivery in the rat are covered. In this example, the purpose is to mimic a disease that affects the entire spinal cord. The widespread transduction can be used to deliver a therapeutic protein to the CNS from a one-time, peripheral administration.
10:32
Surgical Tips and Tricks for Performing Porcine Pancreas Transplantation
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Cited by 4 •
2022
The video article summarizes the technique of pancreatectomy and pancreas allotransplantation in a porcine 3-day survival model with a step-by-step description of the method and emphasis on the surgical tips and tricks to deal with the precarious and delicate porcine visceral anatomy.
FAQs
Cotton fibers create an absorbent contact surface that can retain cells, microorganisms, secretions, or surface residues from a sampled area. When the applicator contacts another destination, such as culture media, a transport tube, a microscope slide, or a test area, the collected material can be released. This capture-and-transfer behavior supports small-scale biological handling.
Suitability depends on the organism being handled and on the requirements of the downstream analysis. The applicator material and handling conditions must allow the sample to be collected and released without undermining its intended examination or use. Choosing appropriately helps preserve sample integrity, whether the next step involves culture, microscopy, staining, transport, or another test area.
Careful handling helps limit contamination and preserve the integrity of the collected material. Unwanted material introduced during sampling or transfer can affect what reaches the culture medium, transport tube, slide, or test area. Consistent technique is therefore important not only for obtaining a sample, but also for ensuring that the transferred material remains suitable for the biological workflow.
A basic workflow starts by bringing the fiber-wrapped tip into contact with the material or surface being sampled. The collected material is then transferred to its intended destination, which may be culture media, a transport tube, a microscope slide, or another test area. The same general sequence supports specimen collection, inoculation, staining, and environmental sampling.
Their use varies with the immediate laboratory purpose. A sample may be collected for examination, moved into culture media, applied during inoculation or staining, or obtained from an environmental surface. These applications rely on the same small-scale transfer capability, but the organism, sample type, and downstream analysis determine the appropriate material and handling conditions.
They are useful when a workflow requires collection or movement of small amounts of biological material, including cells, microorganisms, secretions, or surface residues. Biology teaching laboratories, clinical workflows, and research settings can use them for specimen collection, culture-related handling, staining, or environmental sampling. Their value depends on careful technique and compatibility with the intended analysis.