Cellular Metabolism
Cellular Metabolism - Related Videos
07:36
Autofluorescence Imaging to Evaluate Cellular Metabolism
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Cited by 15 •
2021
This protocol describes fluorescence imaging and analysis of the endogenous metabolic coenzymes, reduced nicotinamide adenine (phosphate) dinucleotide (NAD(P)H), and oxidized flavin adenine dinucleotide (FAD). Autofluorescence imaging of NAD(P)H and FAD provides a label-free, nondestructive method to assess cellular metabolism.
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
04:47
Multimodal Optical Imaging Platform for Studying Cellular Metabolism
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2025
Utilizing a multimodal platform combining label-free optical imaging modalities, we have developed a protocol for visualizing and quantifying cellular dynamics and metabolism. Through imaging via multiphoton fluorescence, second harmonic generation, and stimulated Raman scattering microscopy, we can generate a holistic overview of the cellular and molecular environment.
00:52
What is Metabolism?
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2019
Overview Metabolism represents all of the chemical activity in a cell, including reactions that build molecules (anabolism) and those that break molecules down (catabolism). Anabolic reactions require energy, whereas catabolic reactions provide it. Thus, metabolism describes how cells transform energy through a variety of chemical reactions, which are often made more efficient with the help of enzymes. Metabolism Is the Sum of All of the Chemical Reactions That Take Place in an Organism
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
FAQs
Catabolic pathways break down nutrients, capturing energy in ATP and reduced electron carriers, while anabolic pathways use that energy and precursor molecules to assemble proteins, lipids, nucleic acids, and other biomolecules. Shared intermediates connect the two directions, allowing nutrient breakdown to support cellular construction and helping the cell coordinate energy capture with its biosynthetic demands.
Enzymes regulate each reaction step and help control how material moves through connected pathways. Shared intermediates link multiple routes, allowing products from one process to serve as inputs for another. These regulatory connections help cells maintain internal conditions and respond to environmental demands by coordinating energy-producing breakdown with the synthesis of cellular components.
ATP and reduced electron carriers are forms of energy captured during nutrient breakdown. ATP provides usable energy for processes such as biosynthesis, while reduced electron carriers retain captured energy within the metabolic network. Examining these outputs alongside precursor use helps researchers assess whether cellular activity emphasizes energy acquisition, construction of components, or coordination of both.
Metabolic measurements provide information about cellular activity, while pathway analysis places those observations within the broader reaction network. Together, they can connect changes in energy capture, precursor use, and biomolecule synthesis to biological outcomes. This combined perspective supports research in nutrition, cancer, diabetes, and the identification of potential therapeutic targets without isolating reactions from their pathway context.
Researchers examine cellular metabolism across growth, exercise, development, disease, and microbial activity because these settings place different demands on energy capture and construction of cellular components. A network-level view helps relate pathway activity to those demands and compare biological contexts. It also provides a framework for understanding how cells maintain internal conditions while responding to their environments.
Pathway analysis can relate metabolic activity to disease and nutritional conditions by examining energy capture, precursor use, and biosynthetic activity together. In cancer and diabetes research, this perspective may help connect pathway behavior with broader biological outcomes. In nutrition studies, it supports analysis of how nutrient breakdown and cellular construction are linked, while also informing searches for therapeutic targets.