Journal
/
Bioengineering
/
Calorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Calorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism

Calorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism

DOI:
10.3791/57724-v

•

09:08 min

•

May 31, 2018

•

, ,
1Department of Biological Sciences,University of Louisville, 2Department of Chemistry,Ball State University

Chapters

  • 00:04Title
  • 00:51Respirometer and Colorimeter Preparation
  • 02:25Human Hepatocellular Carcinoma (HepG2) Cell Preparation
  • 04:26Calorimetry and Respirometry
  • 06:36Results: Representative O2 Concentration and Flux Analyses
  • 08:23Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes calorespirometry, the direct and simultaneous measurement of both heat dissipation and respiration, which provides a noninvasive approach to assess energy metabolism. This technique is used to assess the contribution of both aerobic and anaerobic pathways to energy utilization by monitoring the total cellular energy flow.

Tags

Keywords: CalorespirometryCellular Energy MetabolismMitochondrial ToxicityPharmaceutical DevelopmentCryobiologyRespirometerCalorimetryOxygen ConsumptionHeat ProductionHepG2 Cells

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57724?article_type=v

The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal Skin and Melanoma Progression
check_url/57724?article_type=v

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves
check_url/57724?article_type=v

Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering
check_url/57724?article_type=v

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
check_url/57724?article_type=v

Establishment of a Surgically-induced Model in Mice to Investigate the Protective Role of Progranulin in Osteoarthritis
check_url/57724?article_type=v

FIBS-enabled Noninvasive Metabolic Profiling
check_url/57724?article_type=v

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data
check_url/57724?article_type=v

Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix
check_url/57724?article_type=v

An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage
check_url/57724?article_type=v

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Read Article