This protocol describes calorespirometry, the direct and simultaneous measurement of both heat dissipation and respiration, which provides a noninvasive approach to assess energy metabolism. This technique is used to assess the contribution of both aerobic and anaerobic pathways to energy utilization by monitoring the total cellular energy flow.
Skolik, R. A., Konkle, M. E., Menze, M. A. Calorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism. J. Vis. Exp. (135), e57724, doi:10.3791/57724 (2018).