Cooling Stage Assembly
Cooling Stage Assembly - Related Videos
08:23
Assembly and Operation of a Cooling Stage to Immobilize C. elegans on Their Culture Plates
0 Views •
2023
This paper describes protocols for constructing and operating a cooling stage to immobilize C. elegans on their original cultivation plates en masse.
02:44
Heating and Cooling Curves
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2020
When a substance—isolated from its environment—is subjected to heat changes, corresponding changes in temperature and phase of the substance is observed; this is graphically represented by heating and cooling curves. For instance, the addition of heat raises the temperature of a solid; the amount of heat absorbed depends on the heat capacity of the solid (q = mcsolidΔT). According to thermochemistry, the relation between the amount of heat absorbed or released by a substance, q, and its...
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
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Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
11:21
Cooling an Optically Trapped Ultracold Fermi Gas by Periodical Driving
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Cited by 1 •
2017
We present a parametric driving method to cool an ultracold Fermi gas in a crossed-beam optical dipole trap. This method selectively removes high-energy atoms from the trap by periodically modulating the trap depth with frequencies that are resonant with the anharmonic components of the trapping potential.
09:32
Cooling Rate Dependent Ellipsometry Measurements to Determine the Dynamics of Thin Glassy Films
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Cited by 2 •
2016
Here, we present a protocol for cooling rate dependent ellipsometry experiments, which can determine the glass transition temperature (Tg), average dynamics, fragility and the expansion coefficient of the super-cooled liquid and glass for a variety of glassy materials.
FAQs
Cooling begins when the assembly’s cooling element removes heat from the microscope stage. That heat-transfer step lowers the temperature around the specimen, while the stage provides the physical platform for observation. The arrangement links thermal control with microscopy, allowing a biological sample to remain under a deliberately reduced temperature rather than being exposed to uncontrolled conditions during viewing.
Temperature sensors measure the stage condition and provide information to the feedback control system. The controller uses that information to regulate cooling toward the selected set point and limit temperature fluctuations. This control is important because it helps maintain consistent experimental conditions, reducing the chance that changing stage temperature will be mistaken for a biological response.
A defined reduced temperature helps preserve specimen stability while observations or assays are performed. It also establishes a consistent thermal condition against which cellular activity or specimen responses can be examined. Without that controlled reference, environmental temperature variation could affect the sample and make it harder to determine whether an observed change reflects biology or temperature instability.
The researcher places the specimen on the microscope platform, selects the intended reduced temperature, and uses the controlled stage during observation. Sensors and feedback regulation maintain the selected condition while imaging proceeds. This workflow keeps temperature treatment integrated with microscopy, so the sample can be examined without abandoning the thermal conditions required by the experiment.
Controlled cooling supports live-cell imaging, temperature-sensitive assays, and experiments that examine how specimens respond to changing thermal conditions. In each case, the assembly supplies a defined reduced-temperature environment while the biological material is observed. This makes it useful when temperature is an experimental variable or when preserving sample stability is necessary for reliable microscopy.
Temperature control improves imaging consistency and gives researchers a clearer basis for interpreting biological changes. By limiting uncontrolled thermal variation, the experiment can better distinguish effects associated with cellular activity or specimen responses from effects caused by the surrounding conditions. The resulting observations are therefore more closely tied to the intended temperature treatment and experimental design.