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JoVE Journal
Biology

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Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination
 

Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination

Article doi: 10.3791/220
July 4th, 2007

Summary July 4th, 2007

Once a gene is identified as potentially refractory for the dengue virus, it must be evaluated for it's role in preventing viral infections within the mosquito. This protocol illustrates how the extent of dengue infections of mosquitoes can be assayed. The techniques for growing up the virus in culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.

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