Journal
/
Biology
/
Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination

Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination

DOI:
10.3791/220-v

•

15:25 min

•

July 04, 2007

•

, , , ,
1Malaria Research Institute, Bloomberg School of Public Health,Johns Hopkins University

Chapters

  • 00:01Introducton
  • 00:23Critical Reagents
  • 01:05Propagate the Virus in C6/36 Cell Line
  • 02:49Prepare the Mixture of Virus and Blood
  • 04:29Feeding Mosquitoes
  • 07:25Assay of Virus Titer in Mosquito Tissue
  • 11:435 Day Incubation
  • 14:10Conclusion
  • 15:13End Credits

Summary

Automatic Translation

Automatic Translation

Once a gene is identified as potentially refractory for the dengue virus, it must be evaluated for it's role in preventing viral infections within the mosquito. This protocol illustrates how the extent of dengue infections of mosquitoes can be assayed. The techniques for growing up the virus in culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.

Tags

ProtocolDengue InfectionsMosquitoesAedes AegyptiInfection Phenotype DeterminationLaboratory ConditionInfection LevelVirus DynamicsMosquito-virus InteractionsHost FactorsVector CompetenceBSL2 LaboratoryProper AttireGlovesLab Coat75% Ethanol TreatmentBleachingAutoclaving

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/220?article_type=v

Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
check_url/220?article_type=v

Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
check_url/220?article_type=v

Dissection of Midgut and Salivary Glands from Ae. aegypti Mosquitoes
check_url/220?article_type=v

Protocol for RNAi Assays in Adult Mosquitoes (A. gambiae)
check_url/220?article_type=v

Preventing the Spread of Malaria and Dengue Fever Using Genetically Modified Mosquitoes
check_url/220?article_type=v

Building a Better Mosquito: Identifying the Genes Enabling Malaria and Dengue Fever Resistance in A. gambiae and A. aegypti Mosquitoes
check_url/220?article_type=v

RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes
check_url/220?article_type=v

A Multi-detection Assay for Malaria Transmitting Mosquitoes
check_url/220?article_type=v

Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia
check_url/220?article_type=v

A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)

Read Article