Dual Cell Recording
Dual Cell Recording - Related Videos
16:38
Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
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Cited by 19 •
2012
The preparation of acute brain slices from isolated hippocampi, as well as the simultaneous electrophysiological recordings of astrocytes and neurons in stratum radiatum during stimulation of schaffer collaterals is described. The pharmacological isolation of astroglial potassium and glutamate transporter currents is demonstrated.
09:27
Whole Cell Recordings from Brain of Adult Drosophila
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Cited by 40 •
2007
This video demonstrates the procedure for isolating whole brains from adult Drosophila in preparation for recording from single neurons using standard whole cell technology. It includes images of GFP labeled cells and neurons viewed during recording.
09:30
Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices
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Cited by 3 •
2010
Activity in neuronal systems often requires synchronous action potential discharges from neurons within a specific population. For example, pulses of gonadotropin-releasing hormone (GnRH) likely require coordinated activity between GnRH neurons. We present our methodological approach for reliably obtaining simultaneous electrophysiological recordings from the diffusely distributed GnRH neurons.
04:44
Dual Extracellular Recordings in the Mouse Hippocampus and Prefrontal Cortex
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2024
This protocol outlines the use of a custom-designed recording device and electrodes to record local field potentials and investigate information flow in the mouse hippocampus and prefrontal cortex.
11:21
Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
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Cited by 5 •
2010
This protocol describes a general approach to perform photoconversion of fluorescent proteins on a confocal laser scanning microscope. We describe procedures for the photoconversion of puried protein samples, as well as for dual-probe optical highlighting in live cells with mOrange2 and Dronpa.
FAQs
The key evidence for a functional connection is a response in the monitored cell that follows stimulation of the other cell. Researchers compare response timing and properties with the elicited activity, using this relationship to assess whether the cells communicate and to characterize the resulting synaptic interaction. This makes temporal coordination central to interpreting a dual recording.
Connection strength is inferred from the properties of the response produced in the second cell, while the direction and character of the interaction help distinguish excitatory from inhibitory effects. Recording voltage or synaptic currents provides complementary views of how stimulation changes the receiving cell. These measurements connect cellular responses with the functional organization of a neural circuit.
Because both cells are observed during the same experiment, researchers can relate the stimulated cell's activity to the receiving cell's response. This pairing helps separate properties of individual cells from effects of their interaction, supporting analysis of how cellular mechanisms contribute to neural circuits and how those circuits develop.
Researchers establish whole-cell patch-clamp recordings using two electrodes, assign one cell as the stimulated cell, and inject current or elicit action potentials in it. They then monitor the second cell for voltage changes or synaptic currents. Comparing the stimulation with the recorded response provides the basis for assessing communication and describing the connection.
In synaptic transmission studies, the measured response shows how activity in one cell affects another at the cellular level. Researchers can use response timing and properties to characterize the interaction, while connection strength and excitatory or inhibitory effects provide additional descriptors. This links synaptic behavior to the operation of neural circuits rather than treating cells in isolation.
By linking individual cell properties with responses between cells, the method helps researchers examine how cellular mechanisms contribute to circuit function. In developmental studies, these measurements can characterize communication, connection strength, and excitatory or inhibitory interactions as features of neural circuits. The approach therefore connects cellular observations with broader questions about circuit development.