Whole Cell Recordings from Brain of Adult Drosophila

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Biology

JoVE Journal
Biology

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JoVE Journal Biology

Whole Cell Recordings from Brain of Adult Drosophila

DOI:

10.3791/248-v

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09:27 min

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July 29, 2007

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Huaiyu Gu, Diane K. O’Dowd

¹Department of Development and Cell Biology, Department of Anatomy and Neurobiology,University of California, Irvine (UCI)

Chapters

00:04Title
00:22Introduction
00:53Preparation of Dissection Solution
02:15Adult Drosophila Selection
02:32Sex Differentiation
03:04Decapitation and Removal of Brain
04:19Removal of Trachea and Enzyme Treatment
05:27Stabilizing Brain in Recording Chamber
07:58Conclusion

Summary

Automatic Translation

This video demonstrates the procedure for isolating whole brains from adult Drosophila in preparation for recording from single neurons using standard whole cell technology. It includes images of GFP labeled cells and neurons viewed during recording.

Whole Cell Recordings from Brain of Adult Drosophila

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Chapters

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