Embryonic State

Embryonic state refers to the early phase of organismal development after fertilization, when a newly formed embryo undergoes rapid changes that establish its basic body plan. During this period, repeated cell division produces progressively organized cell populations, while cell signaling, migration, and differentiation generate germ layers and begin forming tissues and organs. Understanding the embryonic state is central to developmental biology because it explains how a single cell develops into a complex organism and how disruptions can cause developmental abnormalities. It also supports applications in fertility research, embryonic stem cell studies, regenerative medicine, and the investigation of early disease mechanisms.

Embryonic State - Related Videos

Research

JoVE Journal - Biology

A System for ex vivo Culturing of Embryonic Pancreas

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Cited by 24 •

2012

Here, we describe a method for isolation, culture and manipulation of mouse embryonic pancreas. This represents an excellent ex vivo system for studying various aspects of pancreatic development, including morphogenesis, differentiation and growth. Pancreatic bud explants can be cultured for several days and used in a range of different applications, including whole-mount immunofluorescence and live imaging.

Isolation and Derivation of Mouse Embryonic Germinal Cells

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Cited by 4 •

2009

The ability of embryonic germinal cells to differentiate into primordial germinal cells during early development stages is a perfect model to address our hypothesis about cancer and infertility. This protocol shows how to isolate primordial germinal cells from developing gonads in 10.5-11.5 days post coitum mouse embryos.

Immunostaining of Dissected Zebrafish Embryonic Heart

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Cited by 35 •

2012

A rapid way to conduct immunostaining of zebrafish embryonic heart is described. Compared to the whole mount immunostaining approach, this method dramatically increases the penetration of the antibodies, which allows obtaining high resolution images that reveal cellular/subcellular structures in the heart within a much reduced processing time.

Research

JoVE Journal - Biology
Free Sample

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

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Cited by 170 •

2012

The quality of mouse embryonic fibroblasts (MEFs) is dictated by the right strain of mouse such as CF-1. Pluripotency-supportive MEFs and conditioned media (CM) obtained from these should contain optimal concentrations of Activin A, Gremlin and Tgfβ1 needed for the Activin/Nodal and FGF pathways to co-operatively maintain self-renewal and pluripotency.

Isolation of Embryonic Ventricular Endothelial Cells

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Cited by 4 •

2013

Primary cell culture is a useful technique for analyzing specific populations of cells, particularly from transgenic mouse embryos at specific developmental stages. Herein, embryonic ventricles are dissected and dissociated, and antibody-conjugated beads recognize and separate out the endothelial cells for further analysis.

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