Entamoeba Histolytica
Entamoeba Histolytica - Related Videos
06:05
Development of a Negative Selectable Marker for Entamoeba histolytica
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Cited by 3 •
2010
We report development of a negative selection system in E. histolytica based upon transgenic expression of a chimeric protein (FCU1) and selection with the prodrug 5-fluorocytosine. The FCU1 protein is a fusion of yeast cytosine deaminase and uracil phosphoribosyltransferase. Expression of FCU1 resulted in increased E. histolytica sensitivity towards 5-fluorocytosine.
11:49
Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection
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Cited by 5 •
2014
Human infection by Entamoeba histolytica leads to amoebiasis, a major cause of diarrhea in tropical countries. Infection is initiated by pathogen interactions with intestinal epithelial cells, provoking the opening of cell-cell contacts and consequently diarrhea, sometimes followed by liver infection. This article provides a model to assess the early host-pathogen interactions to improve our understanding of amoebiasis pathogenesis.
12:36
In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
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Cited by 20 •
2010
This protocol describes the preparation of a synthetic tRNA substrate for the Entamoeba histolytica DNA/tRNA methyltransferase 2 (Dnmt2) homolog Ehmeth and the measure of its methyltransferase activity. This experimental approach can be used for investigating the activity of other Dnmt2 proteins.
05:51
Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
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Cited by 11 •
2011
A method for the determination of acetate kinase activity is described. This assay utilizes a direct reaction for determining enzyme activity and kinetics of acetate kinase in the acetate-forming direction with different phosphoryl acceptors. Furthermore, this method can be utilized for assaying other acetyl phosphate or acetyl-CoA utilizing enzymes.
FAQs
Infection begins after environmentally resistant cysts are ingested. Inside the intestine, they release trophozoites, the motile forms associated with attachment and tissue damage. This stage-based transition is central to the parasite’s biology because cysts support environmental persistence and transmission, whereas trophozoites interact directly with intestinal tissues and can contribute to invasive disease.
Trophozoites first attach to colonic cells through adhesion molecules, establishing close contact with host tissue. They also produce proteolytic enzymes that degrade tissue, helping explain how infection can progress from intestinal colonization to damage of the intestinal wall. Studying these components clarifies the molecular basis of host–pathogen interaction and tissue invasion.
The clinical spectrum extends from asymptomatic infection to severe dysentery and liver abscesses, indicating that parasite behavior and host–pathogen interactions influence disease expression. The same organism can remain associated with intestinal infection or cause tissue invasion and extraintestinal disease. This range makes outcome variation an important focus of biological and medical research.
After trophozoites attach to and damage colonic tissue, they may invade the intestinal wall and spread beyond the intestine to the liver. This progression connects local parasite activity with extraintestinal disease, including liver abscesses. Investigating that transition helps researchers examine how tissue invasion and spread shape the most severe outcomes of amoebiasis.
Diagnostic testing helps researchers and healthcare investigations examine whether infection is present in the context of amoebiasis. It is relevant across the disease spectrum, including cases that may be asymptomatic as well as those associated with dysentery or liver abscesses. Testing therefore supports studies of parasite detection, disease patterns, and evaluation of control strategies.
The environmentally resistant cyst stage allows the parasite to persist outside the host long enough to support transmission through ingestion. This feature links parasite biology with public-health conditions, especially settings where sanitation is inadequate. Research on cyst persistence and transmission can inform strategies designed to reduce exposure and limit the spread of infection.
Research commonly connects several levels of investigation: parasite development from cysts to trophozoites, attachment to colonic cells, tissue degradation, invasion, and spread to the liver. These studies also support diagnostic testing and antiparasitic treatment research. Together, they provide a framework for understanding parasite biology, host–pathogen interactions, disease transmission, and approaches to reducing amoebiasis.