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Immunology and Infection

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JoVE Journal Immunology and Infection
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Development of a Negative Selectable Marker for Entamoeba histolytica

Article DOI: 10.3791/2410 • 06:05 min
December 12th, 2010 •
1, 1, 1, 1
1Division of Infectious Disease and International Health, University of Virginia Health System

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December 12th, 2010

We report development of a negative selection system in E. histolytica based upon transgenic expression of a chimeric protein (FCU1) and selection with the prodrug 5-fluorocytosine. The FCU1 protein is a fusion of yeast cytosine deaminase and uracil phosphoribosyltransferase. Expression of FCU1 resulted in increased E. histolytica sensitivity towards 5-fluorocytosine.

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