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Development of a Negative Selectable Marker for Entamoeba histolytica
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Chapters
- 00:05Title
- 00:58Introduction
- 01:37FCU Negative Selection System in Entamoeba histolytica
- 03:23Determination of Selection Efficiency
- 04:38The FCU1/5-FC System: A Powerful Negative Selection Tool for E. histolytica Trophozoites
- 05:43Conclusion
We report development of a negative selection system in E. histolytica based upon transgenic expression of a chimeric protein (FCU1) and selection with the prodrug 5-fluorocytosine. The FCU1 protein is a fusion of yeast cytosine deaminase and uracil phosphoribosyltransferase. Expression of FCU1 resulted in increased E. histolytica sensitivity towards 5-fluorocytosine.
Tags
Negative Selectable MarkerEntamoeba HistolyticaAmebiasisGene ExpressionTransfectionPlasmidsReverse GeneticsHomologous RecombinationEpisomal Plasmid DNABackground Of Exogenous DNAGenomic Integration EventNegative Selection SystemYeast Cytosine Deaminase And Uracil Phosphoribosyl Transferase Chimera (FCU1)Prodrug 5-fluorocytosine (5-FC)5-fluorouracil5-fluorouridine-5′-monophosphate
