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JoVE Journal Biology
Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
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Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Article DOI: 10.3791/2561
March 31st, 2011 •
1, 1, 1
1Department of Cancer Biology, University of Massachusetts Medical School

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March 31st, 2011

A rapid, robust way of isolating viable adult epithelial stem cells from human skin is described. The method utilizes enzymatic digestion of skin collagen matrix , followed by plucking of hair follicles and isolation of single cell suspensions or tissue fragments for cell culture.

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