Falcon Culture Plate
Falcon Culture Plate - Related Videos
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The floor plate is a crucial structure of the developing spinal cord, providing diffusible signals to progenitors and differentiating neurons. We describe a method to produce floor-plate conditioned medium, to apply it to fresh spinal cord tissue, and to assess the consequences on proteins of interest by biochemistry.
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Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System
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The protocols described allow laboratories to perform scalable, adherent stem cell culture in high throughput with minimal labor, experience and equipment investment cost using a programmable liquid handling robot and 96-well plates. iPSCs passaged more than 20 times on this system maintained pluripotency, normal karyotypes and differentiated into cardiomyocytes.
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Pure Cultures and Streak Plating: Isolation of Single Bacterial Colonies from a Mixed Sample
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Source: Tilde Andersson1, Rolf Lood1 1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund, Sweden Seemingly impossible to determine, microbial biodiversity is truly astounding with an estimated one trillion coexisting species (1,2). Although particularly harsh climates, like the acidic environment of the human stomach (3) or the subglacial lakes of Antarctica (4), may be dominated by a specific species, bacteria are typically...
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Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
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Cited by 9 •
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This protocol describes a cell transplantation system using genetically modified, injectable spheroids. Cell spheroids are cultured on micropatterned culture plates and recovered after gene introduction using polyplex nanomicelles. This system facilitates prolonged transgene expression from the transplanted cells in host animals while maintaining the innate function of the cells.
FAQs
The treated surface promotes attachment and spreading of anchorage-dependent cells, which need a surface to remain organized during culture. This property makes the plate suitable for maintaining cells through incubation rather than merely holding a liquid sample. Consistent attachment also supports microscopy and downstream comparisons in which cell appearance or behavior is evaluated across wells.
Each well holds a defined volume of culture medium, giving researchers separate spaces for handling cells and comparing conditions. Because many wells are available on one plate, samples can be treated or observed in parallel while conserving culture materials and reagents. This layout supports organized, reproducible comparisons within the same experimental vessel.
Attachment keeps anchorage-dependent cells associated with the treated surface during incubation, allowing their spreading to be examined in the well. This arrangement lets microscopy capture cell appearance and behavior in the same culture environment used for comparisons. It therefore connects the plate’s surface property with visual analysis of cultured cells.
Researchers allocate cells and defined culture-medium volumes among wells, incubate the plate under the selected experimental conditions, and then examine or measure the cultures. Depending on the study, analysis may involve microscopy, viability assessment, drug-response testing, or comparisons among wells. Parallel handling helps organize these steps within one experiment.
It is useful when a study must compare multiple experimental conditions, such as different drug-response settings, in parallel. Separate wells allow cultures to be handled and analyzed individually while conserving samples and reagents. The same format can support viability assays or microscopy, so researchers can connect treatment conditions with observable or measured cellular outcomes.
They provide a controlled, parallel format for examining cell behavior and for performing assays relevant to disease mechanisms and therapeutic development. Researchers can maintain cultures, inspect them by microscopy, and compare viability or drug responses across wells. These capabilities make the plates useful for linking cellular observations to broader biological or biomedical questions.