Genetic Recombination
Genetic Recombination - Related Videos
09:29
Genetic Modification and Recombination of Salivary Gland Organ Cultures
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Cited by 20 •
2013
A technique to genetically manipulate epithelial cells within whole ex vivo cultured embryonic mouse submandibular glands (SMGs) using viral gene transfer is described. This method takes advantage of the innate ability of SMG epithelium and mesenchyme to spontaneously recombine after separation and infection of epithelial rudiments with adenoviral vectors.
04:07
Genetic Modification of Primary Human Keratinocytes: A Method for Genetically Manipulating Keratinocytes Using Recombinant Retroviruses
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2023
This video describes a detailed protocol for producing genetically modified primary human keratinocytes recombinant retrovirus. This method can be used to manipulate gene expression in keratinocytes.
12:13
TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
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Cited by 32 •
2012
A quick method to modify the genome of V. cholerae is described. These modifications include the deletion of single genes, gene clusters and genomic islands as well as the integration of short sequences (e.g. promoter elements or affinity-tag sequences). The method is based on the natural transformation and FLP-recombination.
08:56
Recombineering and Gene Targeting
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2023
One of the most widely used tools in modern biology is molecular cloning with restriction enzymes, which create compatible ends between DNA fragments that allow them to be joined together. However, this technique has certain restrictions that limit its applicability for large or complex DNA construct generation. A newer technique that addresses some of these shortcomings is recombineering, which modifies DNA using homologous recombination (HR), the exchange between different DNA molecules based...
10:38
An Overview of Genetic Engineering
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2023
Genetic engineering – the process of purposefully altering an organism’s DNA – has been used to create powerful research tools and model organisms, and has also seen many agricultural applications. However, in order to engineer traits to tackle complex agricultural problems such as stress tolerance, or to realize the promise of gene therapy for treating human diseases, further advances in the field are still needed. Important considerations include the safe and efficient delivery of genetic...
FAQs
During meiosis, crossing over exchanges corresponding DNA segments between paired homologous chromosomes, whereas independent assortment reshuffles whole homologous chromosomes into different combinations. These are distinct sources of genetic variation: one changes the arrangement of segments within chromosome pairs, and the other changes which chromosome copies enter resulting cells. Considering both helps explain allele combinations and chromosome behavior.
DNA break repair can rearrange or restore genetic material, linking recombination to genome integrity rather than variation alone. Molecular repair processes handle damaged or broken DNA, while the resulting exchange or rearrangement can create new allele combinations. Studying this relationship helps biology connect chromosome maintenance with genetic diversity and investigate genetic disease.
Horizontal gene transfer introduces genetic material between organisms, providing a route distinct from meiotic exchange between homologous chromosomes. Its importance is that recombination is not limited to inheritance through chromosome pairing: genetic material can also be acquired and rearranged through other biological processes. Comparing these routes broadens analyses of diversity, genome organization, and evolutionary change.
Because recombination creates new combinations of alleles, researchers can use recombination studies to support gene mapping and analyze inheritance. These investigations also examine how genetic material is arranged and transmitted, connecting observed variation with chromosome behavior. This makes recombination a practical framework for relating inherited patterns to genome organization and chromosome-level processes.
In crop and animal breeding, researchers consider genetic recombination because it generates new combinations of alleles within inherited material. These new combinations provide variation for breeding studies and help investigators analyze inheritance. The application connects chromosome-level events with practical efforts to understand and develop organisms carrying particular genetic combinations.
Research on genetic recombination can reveal how chromosome behavior and genome organization relate to genetic disease. Investigators can examine recombination alongside DNA break repair and the maintenance of genome integrity, then connect those processes with altered genetic material. This context makes the topic useful not only for studying diversity and evolution, but also for understanding disease-related genetic mechanisms.