Glycerol Metabolism
Glycerol Metabolism - Related Videos
02:35
Long-Term Cryopreservation of Cyanobacterial Strains Using Glycerol or Dimethyl Sulfoxide
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2025
Source: Lea-Smith, D. J., et al. Generation of Marked and Markerless Mutants in Model Cyanobacterial Species. J. Vis. Exp. (2016)This video demonstrates the cryopreservation of cyanobacterial strains by combining concentrated cell suspensions with glycerol or DMSO and storing them at low temperatures, enabling long-term maintenance of viable cultures for photosynthesis and genetic studies.
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
13:02
Archimedes-Based Glycerol Displacement for Electrode Porosity Measurement in Lead-Acid Batteries
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2026
Here, we present a simple and low-cost glycerol displacement method based on Archimedes' principle for measuring lead-acid battery electrode porosities. The method demonstrates good reproducibility and is safer than traditional techniques, requiring low-hazard chemicals and equipment. Combining the technique with microstructural analysis by X-ray diffraction provides information on battery electrode properties and degradation.
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
08:28
Metabolic Labeling
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2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
FAQs
Glycerol kinase performs the first specified conversion by phosphorylating glycerol to produce glycerol-3-phosphate. This step prepares glycerol for further processing rather than directing it directly into glycolysis or gluconeogenesis. Its activity therefore connects glycerol released from triglyceride breakdown with the downstream reactions that make this carbon source metabolically useful.
Glycerol-3-phosphate dehydrogenase converts glycerol-3-phosphate into dihydroxyacetone phosphate, or DHAP. Because DHAP is an intermediate shared by glycolysis and gluconeogenesis, this conversion provides a biochemical connection between lipid-derived glycerol and pathways associated with energy production or glucose synthesis. The position of DHAP allows glycerol-derived carbon to participate in either metabolic direction.
The formation of dihydroxyacetone phosphate gives glycerol-derived carbon access to established carbohydrate metabolism. Through this intermediate, glycerol can contribute to glycolysis, which supports cellular energy production, or to gluconeogenesis, which supports glucose synthesis. This dual connection explains how glycerol metabolism participates in the broader integration of carbohydrate and fat metabolism.
During fasting, glycerol metabolism becomes relevant because triglyceride breakdown releases glycerol while the body adjusts its energy balance. The liver can process this glycerol through the pathway leading to dihydroxyacetone phosphate, linking stored lipid mobilization with either energy-producing metabolism or glucose synthesis. Thus, the pathway helps coordinate fuel use when nutrient availability is reduced.
The liver is identified as a key tissue for processing glycerol released during triglyceride breakdown. By converting glycerol through glycerol-3-phosphate and dihydroxyacetone phosphate, it links lipid-derived material to pathways for energy production and glucose synthesis. This hepatic role makes glycerol metabolism relevant to how the body manages fuel sources after stored triglycerides are mobilized.
Research on glycerol metabolism helps examine how lipid storage connects with carbohydrate use and energy balance. The pathway is relevant to studies of diabetes and metabolic disease because it provides a framework for examining glycerol handling, triglyceride breakdown, and glucose synthesis together. It also supports investigation of how lipid and carbohydrate pathways are integrated across biological conditions.