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Chapters
- 00:05Title
- 02:39Manual Quantitative Fitness Analysis Protocol: Culturing Yeast Strains
- 03:11Fully Automated Quantitative Fitness Analysis Protocol
- 04:48Image and Experimental Metadata Capture: Automated Quantitative Fitness and Data Analysis
- 09:32Representative Fitness Analysis Results
- 10:40Conclusion
Quantitative Fitness Analysis (QFA) is a complementary series of experimental and computational methods for estimating microbial culture fitnesses. QFA estimates the effect of genetic mutations, drugs or other applied treatments on microbe growth. Experiments scaling from focussed analysis of single cultures to thousands of parallel cultures can be designed.
Tags
Quantitative Fitness AnalysisQFAFitness ComparisonMicrobial CulturesGenetic InteractionDrug ScreensGrowth CurvesQuantitative Cell Density EstimatesFitness MeasuresGenetic Interaction StrengthsDrug SensitivitiesSubstrate AgarSmall MoleculesAntibioticsNutrientsUV IrradiationTemperatureGrowth Rate EstimatesSpectrophotometric MeasurementHigh Throughput
