Iliac Crest Harvest

Iliac crest harvest is a surgical technique for obtaining autologous bone graft from the iliac crest, the broad upper border of the pelvic bone, for use in reconstructive procedures. Surgeons expose the crest and remove cortical, cancellous, or corticocancellous bone, which retains the patient’s own viable cells and natural osteogenic, osteoinductive, and osteoconductive properties. The graft can support bone healing in spinal fusion, fracture repair, and oral or maxillofacial reconstruction, where it may promote structural stability and new bone formation. Understanding the anatomy, harvest approach, and potential donor-site complications is essential for safe biological and clinical application.

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Research

JoVE Journal - Medicine

The Rabbit Model of Accelerated Atherosclerosis: A Methodological Perspective of the Iliac Artery Balloon Injury

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Cited by 16 •

2017

Animal models of atherosclerosis are essential to understand the mechanism and to investigate newer approaches to prevent plaque development or rupture, a leading cause of death in the industrialized world. This protocol uses a combination of balloon injury and cholesterol rich diet to induce atherosclerotic plaques in rabbit iliac artery.

Intra-iliac Artery Injection for Efficient and Selective Modeling of Microscopic Bone Metastasis

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Cited by 43 •

2016

This manuscript provides the detailed procedure of intra-iliac artery (IIA) injection, a technique to deliver cancer cells specifically to hind limb tissues including bones to establish experimental bone metastases. Although initially established with breast tumor models, this protocol can be easily extended to other cancer types.

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation

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Cited by 10 •

2012

An approach for analyzing migration and eventual fate of avian neural crest cells in quail-chick chimeric embryos is described. This method is a simple and straightforward technique for tracing neural crest cells during migration and differentiation that are otherwise difficult to distinguish within an unmanipulated chick embryo.

Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay

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Cited by 5 •

2012

An approach to analyze the migration of explanted cells (trunk neural crest cells) is described. This method is inexpensive, gentle, and capable of distinguishing chemotaxis from both chemokinesis and other influences on migratory polarity such as those derived from cell-cell interactions within the primary trunk neural crest cell culture.

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

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Cited by 26 •

2012

Isolation of embryonic neural crest from the neural tube facilitates the use of in vitro methods for studying migration, self-renewal, and multipotency of neural crest.

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