Intracellular Electrode
Intracellular Electrode - Related Videos
03:02
Standard Electrode Potentials
0 Views •
2020
On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...
07:18
Intracellular Refolding Assay
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Cited by 9 •
2012
In this protocol a method to measure intracellular protein refolding after heat shock is described. This method can be used to study foldases like molecular chaperones and their co-factors or compounds able to influence their activity. Firefly luciferase activity is used as reporter to measure chaperone refolding activity.
02:36
In Vivo Recording of Intracellular Signals from a Single Neuron
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2025
This video demonstrates the recording of electrical signals from single brain neurons using a fine-tipped glass micropipette and a specialized setup. The micropipette is carefully inserted into the rat's brain and is buzzed to penetrate the neuron to record internal neuronal electrical signals.
08:29
Fabrication of Amperometric Electrodes
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Cited by 8 •
2009
This protocol describes how to generate carbon fiber electrodes. The electrodes are subsequently used to detect catecholamine release from vesicles with carbon fiber amperometry.
09:51
Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia
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Cited by 4 •
2012
We demonstrate how changes in the intracellular free calcium concentration and synaptic efficacy can be simultaneously monitored in a ganglion preparation of Aplysia. We image intracellular calcium using a fluorescent dye, Calcium Orange, and induce and monitor synaptic transmission with sharp (intracellular) electrodes.
FAQs
The intracellular probe records electrical conditions on the inside of the cell, while a reference electrode remains outside. Comparing these two positions produces a voltage difference across the membrane rather than an isolated electrical value. This arrangement lets researchers examine resting potentials and changes associated with cellular excitation in living biological preparations.
The same recording arrangement can deliver a controlled current into the cell as well as measure voltage. Researchers can therefore examine how the cell responds to an imposed electrical change, not only observe spontaneous activity. This approach helps relate an applied stimulus to excitability and to the resulting membrane-voltage response.
Patterns in membrane potential can distinguish relatively stable resting conditions from rapid electrical events, including action potentials and synaptic responses. These signals show how a cell participates in communication and indicate changes in excitability. Intracellular measurements can also provide information about ion-channel activity, linking electrical behavior with underlying cellular processes.
A researcher positions a sharp microelectrode or patch pipette at the cell, brings its tip across the membrane, and records voltage relative to an external reference electrode. The setup may then inject controlled current and monitor the response. Recorded traces can be examined for resting potentials, action potentials, synaptic responses, or ion-channel activity.
These recordings are especially useful in neurons, muscle cells, and other excitable tissues where membrane-voltage changes carry functional information. They can reveal how cells generate electrical signals and communicate with one another. The resulting measurements support investigations of cellular communication and the relationship between electrical activity and broader biological behavior.
By directly measuring electrical activity in cells, researchers can examine how cellular signaling changes during disease-related investigations or after exposure to a drug. The recordings connect altered membrane behavior with excitability and communication. They also support studies of development, helping relate electrical properties to changing cellular functions and biological states.