L-selectins
L-selectins - Related Videos
01:25
Selectins
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2023
Cell adhesion is an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...
14:16
Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium
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Cited by 32 •
2009
This report provides a visual depiction of parallel-plate flow chamber analysis for studying leukocyte endothelial interactions under physiologic shear stress. This method is particularly useful for investigating the role of endothelial (E)-selectin and leukocyte E-selectin ligands that trigger leukocyte rolling on endothelial cell surfaces.
11:56
In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells
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Cited by 2 •
2014
Our report describes a unique method to visualize and analyze CTC/EC interactions in prostate cancer under physiological flow conditions.
09:39
In-situ Tapering of Chalcogenide Fiber for Mid-infrared Supercontinuum Generation
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Cited by 5 •
2013
We describe a method for in-situ tapering of As2S3 fibers to achieve efficient mid-infrared supercontinuum generation. By tapering while monitoring the supercontinuum’s spectrum, the spectral width can be maximized for a fiber taper. In-situ fiber tapering can be applied to optimize the performance of other fiber-based devices.
09:58
A Method for Labeling Vasculature in Embryonic Mice
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Cited by 14 •
2011
This article describes a method for labeling embryonic skin and thymus blood vessels.
FAQs
Recognition depends on sialylated and fucosylated carbohydrate structures displayed on vascular addressins. When these structures engage L-selectins, the interaction can form brief attachments rather than an immediate permanent arrest. That binding behavior is important because it allows leukocytes to make contact with vessel walls while blood continues to flow, initiating the sequence that supports directed immune-cell recruitment.
Rolling slows leukocytes and keeps them in repeated contact with the vessel wall, creating an opportunity for later, firmer adhesion and tissue entry. Without this transitional behavior, circulating cells would have less opportunity to interact with vascular addressins in the correct location. The process therefore links bloodstream transport with organized migration into lymphoid tissues or inflamed sites.
Enzymatic shedding removes L-selectin from the leukocyte surface, providing a way to regulate the cell’s adhesive state after activation or during migration. Because surface availability influences interactions with vascular addressins, shedding can alter how leukocytes navigate vessels and proceed toward tissue entry. This regulation helps connect leukocyte activation with changes in trafficking behavior.
A study can evaluate whether leukocytes bind sialylated and fucosylated structures on vascular addressins, then examine tethering and rolling under flowing conditions. Researchers can also assess whether L-selectin is enzymatically shed as cells become activated. Together, these observations connect molecular recognition, movement along vessel walls, and regulation of subsequent leukocyte migration.
L-selectin provides a molecular link between circulating white blood cells and the anatomical locations where immune responses are organized. Its interactions with vascular addressins help explain lymphocyte homing to lymphoid tissues and recruitment of leukocytes to inflammatory sites. Studying these events therefore clarifies how immune cells are distributed and directed rather than remaining randomly positioned.
Leukocyte trafficking influences which immune cells reach lymphoid tissues or inflamed locations, so altered L-selectin activity can affect the organization of immune responses. This makes the molecule relevant to studies of infection, chronic inflammation, and autoimmune disease. Research may also explore therapies that modify leukocyte trafficking by changing adhesion or related migration-regulating processes.