Mammalian Cancer Cells
Mammalian Cancer Cells - Related Videos
09:43
Measurement of Heme Synthesis Levels in Mammalian Cells
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Cited by 12 •
2015
Altered intracellular heme levels are associated with common diseases such as cancer. Thus, there is a need to measure heme biosynthesis levels in diverse cells. The goal of this protocol is to provide a fast and sensitive method to measure and compare the levels of heme synthesis in different cells.
07:15
Anaerobic Growth and Maintenance of Mammalian Cell Lines
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Cited by 4 •
2018
Here, we describe a new method that enables the anaerobic long-term cultivation of established cell lines. The maximum survival time that was tested is 17 days. This method is suitable for the testing of cytotoxic agents and exploring the physiology of anoxically replicating cells.
15:31
MISSION esiRNA for RNAi Screening in Mammalian Cells
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Cited by 15 •
2010
Here we use a human esiRNA library in a high-throughput screen for genes involved in cell division. We demonstrate how to set up and conduct an esiRNA screens, as well as how to analyze and validate the results.
00:09
Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines
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2019
The presence of cancer stem cells have been associated with relapse or poor outcomes after radiotherapy. This manuscript describes the methods to study the radiosensitivity of cancer stem cells in lung cancer cell lines.
02:52
Expansion of Mutant Vaccinia Virus in Mammalian Cells
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2026
Source: Yuan, M., et al. A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors. J. Vis. Exp. (2016)This video demonstrates the expansion of plaque-derived recombinant virus in adherent mammalian cells. The virus carries a fluorescent reporter in place of a virulent gene, enabling infection tracking. Infected cells are harvested for PCR verification of the genetic modification.
FAQs
Cell-cycle checkpoints normally help regulate whether a cell proceeds through division, while apoptosis removes cells that are damaged or no longer viable. When these controls are disrupted, mammalian cancer cells can continue proliferating and avoid programmed cell death. Studying both failures helps researchers connect abnormal cell survival with tumor initiation and evaluate therapies designed to restore growth control or promote cell elimination.
Defective DNA repair can allow genomic damage to persist as cells divide. In mammalian cancer cells, this condition helps researchers examine how accumulated abnormalities contribute to tumor progression and treatment responses. It also provides a mechanistic link between damaged genetic information, altered cell behavior, and the need to evaluate therapies that target cancer cells with particular repair vulnerabilities.
Abnormal growth-factor signaling can encourage continued proliferation even when normal regulatory signals would limit cell division. Examining these signaling changes helps explain how mammalian cancer cells respond to their surroundings and maintain expansion. This research is especially relevant to targeted therapies, which are evaluated for their ability to interfere with signaling processes that support abnormal growth.
Cancer-cell behavior is influenced not only by internal genetic and epigenetic changes but also by interactions with the surrounding microenvironment. Studying these interactions helps researchers investigate how cells survive unfavorable conditions, invade tissue, and progress toward metastasis. This broader view connects cell-intrinsic mechanisms with the local biological context that can shape tumor development and treatment outcomes.
Researchers study mammalian cancer cells in culture and in experimental organisms to examine cancer biology at different levels. Culture systems support direct investigation of cellular mechanisms and responses, whereas experimental organisms provide a setting for studying tumor progression, tissue invasion, metastasis, and environmental interactions. Together, these approaches connect controlled cellular observations with more complex biological behavior.
Mammalian cancer cells provide experimental systems for screening potential drugs and evaluating targeted or combination therapies. Researchers can examine how candidate treatments affect abnormal proliferation, survival, or signaling, while biomarker evaluation can help relate cellular features to treatment response. These applications support the broader development of strategies for cancer diagnosis and treatment.