Molecular Technique Detection
Molecular Technique Detection - Related Videos
08:56
In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
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Cited by 4 •
2012
We demonstrate the assembly and application of a molecular-scale device powered by a topoisomerase protein. The construct is a bio-molecular sensor which labels two major types of DNA breaks in tissue sections by attaching two different fluorophores to their ends.
11:28
Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
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Cited by 175 •
2015
The preparing and processing of dried blood spots (DBS) for their final analysis are still poorly standardized for most diagnostic applications. To overcome this shortcoming, a comprehensive step-by-step protocol is suggested and subsequently evaluated with regard to its effectiveness for detecting markers of viral infections.
05:38
Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
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Cited by 4 •
2017
We describe a rapid, molecular-based Influenza A and B assay. The Influenza assay detects each target within 15 min by employing isothermal amplification with influenza-specific primers followed by target detection with molecular beacon probes. The Influenza A and B assay is user-friendly and required minimal hands-on time to perform.
06:54
MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent
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Cited by 3 •
2013
To demonstrate MR cancer molecular imaging with a small peptide targeted MRI contrast agent specific to clotted plasma proteins in tumor stroma in a mouse prostate cancer model.
09:55
Molecular Cloning
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2023
Molecular cloning is a set of methods, which are used to insert recombinant DNA into a vector - a carrier of DNA molecules that will replicate recombinant DNA fragments in host organisms. The DNA fragment, which may be a gene, can be isolated from a prokaryotic or eukaryotic specimen. Following isolation of the fragment of interest, or insert, both the vector and insert must be cut with restriction enzymes and purified. The purified pieces are joined together though a technique called...
FAQs
Selectivity comes from matching the detection reagent to a particular molecular target. Complementary probes recognize DNA or RNA sequences, antibodies recognize proteins, and amplification increases the detectable signal from a target sequence. Because these interactions are target-directed, the resulting fluorescent, electrical, or sequencing-based readout can reveal molecular differences that may not produce visible changes at the cellular or organismal level.
Sensitivity determines how effectively a method can reveal molecular changes, whereas specificity concerns how selectively it identifies the intended target. Their combination is important because a detectable signal is most useful when it corresponds to the relevant DNA, RNA, protein, or other biomolecule. In biological studies, this supports confident links between molecular measurements and disease, development, or biological function.
The target determines which recognition strategy is appropriate and what biological information the measurement can address. DNA or RNA analysis can use complementary probe binding or sequence amplification, while protein analysis can rely on antibody recognition. Selecting among these targets allows researchers to examine genetic material, gene-expression-related changes, or biomarker levels according to the biological question.
A molecular detection workflow generally connects a biological sample to a target-specific recognition step and then to signal measurement. The method may use probe binding, antibody recognition, or amplification of a target sequence. Detection can produce a fluorescent, electrical, or sequencing-based readout, which researchers interpret to identify or measure molecular changes in the sample.
Researchers can apply molecular detection to identify pathogens, perform genetic testing, analyze gene expression, and measure biomarkers. These uses extend across questions about disease, development, and biological function because molecular measurements can expose changes that remain undetectable at the cellular or organismal level. The same general approach can therefore support both identification and measurement of biological targets.
Its applications include environmental monitoring as well as pathogen identification, genetic testing, gene-expression analysis, and biomarker measurement. Environmental use broadens molecular detection from questions about organisms and disease to assessment of biological changes in environmental samples. Across these settings, fluorescent, electrical, or sequencing-based signals provide measurable evidence of selected molecular targets.