Mouse Brain Lesion
Mouse Brain Lesion - Related Videos
04:09
Isolating Microglia from Mouse Brain Demyelinating Lesions Using Magnetic Activated Cell Sorting
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2025
This video demonstrates a protocol for extracting microglial cells from demyelinating lesions in mouse brains using magnetic cell sorting. The protocol involves dissecting the lesioned brain tissue containing microglial cells, treating the tissue with specific enzymes to obtain a single-cell suspension, and utilizing microglial receptor-specific magnetic beads to isolate the microglial cells.
12:45
Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat
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Cited by 22 •
2012
Targeted ablation of specific brain region(s) by infusion of an excitotoxin using stereotaxic coordinates is described. This technique could also be adapted for infusion of other chemicals into the rat brain.
03:35
Contrast-Enhanced Micro-CT Imaging of Cerebral Cavernous Malformation Lesions in a Mouse Brain
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2025
Source: Choi, J. P. et al. Induction and Micro-CT Imaging of Cerebral Cavernous Malformations in Mouse Model. J. Vis. Exp. (2017)This video demonstrates the use of micro-CT imaging to visualize cerebral cavernous malformation (CCM) lesions in a mouse brain. It details the steps for sample preparation, imaging, and 3D image reconstruction to evaluate the density and location of CCM lesions in brain tissue.
10:09
Development of a Unilaterally-lesioned 6-OHDA Mouse Model of Parkinson's Disease
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Cited by 121 •
2012
A protocol for performing unilateral 6-OHDA lesions of the medial forebrain bundle in mice is described. This method has a low mortality rate (13.3 %) with 89% of the surviving animals showing >95% loss of striatal dopamine and 90.63±-4.02 % ipsiversive rotational bias towards the side of the lesion.
08:40
Photothrombotic Ischemia: A Minimally Invasive and Reproducible Photochemical Cortical Lesion Model for Mouse Stroke Studies
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Cited by 226 •
2013
Photothrombosis is a quick, minimally-invasive technique for inducing small and well-delimited infarction in areas of interest in highly reproducible manner. It is particularly suitable for studying cellular and molecular responses underlying brain plasticity in transgenic mice.
FAQs
The key inference comes from comparing mice with affected tissue against appropriate controls while measuring behavior and physiology. If the groups differ, the findings can associate the damaged region with particular functions or responses. This comparative design helps connect neural structure with function while reducing the risk that normal variation will be mistaken for a lesion-related effect.
These approaches produce or represent neural damage through different experimental conditions. Targeted surgical injury directly affects a selected area, chemical disruption provides another way to disturb tissue, and disease models reproduce lesion-related changes in a disease context. Choosing among them allows investigators to examine brain dysfunction from structural, chemical, or disease-related perspectives.
Each measurement captures a different consequence of the lesion. Behavioral tests assess changes in observable function, imaging helps examine the brain in the experimental context, and histological analysis evaluates tissue-level changes. Together, these readouts provide complementary evidence for interpreting how damage affects neural circuits rather than relying on a single outcome.
A typical study first creates or identifies the lesion using a targeted injury, chemical disruption, or disease model. Researchers then include appropriate control mice, assess behavior or physiology, and examine the brain with imaging and histological analysis. Comparing these results allows the affected region and its functional consequences to be evaluated systematically.
Results may show how a particular brain area relates to behavior, physiology, or the operation of a neural circuit. Structural findings from tissue analysis can be considered alongside functional changes detected in testing. This combination helps characterize brain dysfunction and clarifies whether observed effects correspond with the affected neural region.
Lesion studies can model aspects of neurological disease and provide a framework for examining the mechanisms of brain dysfunction. When combined with genetic and molecular tools, they can help connect tissue damage with underlying biological processes. These findings may also support the development of therapeutic strategies by identifying affected circuits and measurable outcomes.