Myeloid Lineage
Myeloid Lineage - Related Videos
07:21
Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
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Cited by 12 •
2018
We demonstrate how to identify and isolate 6 subsets of myeloid progenitors from murine bone marrow using a combination of magnetic and fluorescence sorting (MACS and FACS). This protocol can be used for in vitro culture assays (methylcellulose or liquid cultures), in vivo adoptive transfer experiments, and RNA/protein analyses.
06:39
Culture of myeloid dendritic cells from bone marrow precursors
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Cited by 22 •
2008
This video demonstrates the procedure for differentiating myeloid dendritic cells from mouse bone marrow. Isolation of mouse tibia and femur, and processing of bone marrow are demonstrated. Pictures demonstrating cell morphology before and after differentiation, and figures depicting cell phenotype and IL-12 production following maturation using CpG are shown.
01:21
Lineage Commitment
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2023
Commitment is the process whereby stem cells: lose their ability to form all cell types and irreversibly change into a specific type. The multipotent hematopoietic stem cells, (HSCs), differentiate into the multipotent hematopoietic progenitor cells, (HPCs). The HPCs express many lineage-specific cytokine receptors. Each of these receptors binds specific cytokines, activates distinct signaling pathways, and expresses a particular gene set. The HPCs further differentiate to form committed...
07:58
Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood
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Cited by 12 •
2012
We describe use of ImageStream technology (www.amnis.com), which combines quantitative flow cytometry with simultaneous high-resolution digital imaging, to quantify cellular mechanisms of primary immune cells from well-defined patient cohorts. Our studies provide a blueprint for translational investigations to quantify lineage specific cellular responses in small samples from subject cohorts.
05:14
Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo
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2025
Given the limited xenograft models available to study interactions between human CART and myeloid cells, we established in vitro and in vivo models to understand the impacts of human macrophages on CART cells. Findings can potentially be generalized to evaluate macrophage roles in the tumor microenvironment and test macrophage-targeted immunotherapies.
FAQs
Commitment occurs progressively rather than in a single step. Hematopoietic stem cells first give rise to myeloid progenitors, and those progenitors respond to lineage-specific transcription factors and cytokine signals that guide subsequent differentiation. This staged control allows one developmental pathway to produce cells with very different roles, including oxygen transport, clot formation, and innate immune defense.
Transcription factors and cytokines provide complementary controls over blood-cell development. Lineage-specific transcription factors help direct progenitors toward particular developmental outcomes, while cytokine signals influence how those cells proceed through differentiation. Studying both inputs helps explain how myeloid progenitors produce distinct erythrocyte, megakaryocyte, granulocyte, monocyte, macrophage, and dendritic-cell populations.
Within the developmental sequence, hematopoietic stem cells represent the starting population, whereas myeloid progenitors are a later, more committed stage. That distinction matters conceptually and experimentally: stem-cell studies address the source of blood-cell development, while progenitor studies focus more directly on lineage-directed differentiation and the signals shaping downstream cellular outcomes.
Myeloid-lineage biology connects disrupted development with specific disease contexts. Problems affecting erythrocyte production are relevant to anemia, whereas altered generation or function of innate immune cells relates to immune dysfunction. Abnormal lineage development is also important in leukemia, so researchers can examine how changes in blood-cell development disturb normal production and cellular roles.
In hematopoietic stem-cell transplantation, knowledge of myeloid differentiation provides a framework for understanding how transplanted stem cells may reestablish blood production. Researchers must consider how effectively their progeny commit and mature along myeloid routes, because those outcomes relate to restoring oxygen-carrying cells, platelet production, and innate immune functions.
Following development from stem cells through myeloid progenitors and mature cell types allows investigators to compare normal and abnormal hematopoiesis. They can ask whether a problem involves progressive commitment, signal-directed differentiation, or the functions of resulting blood cells. This approach supports distinct investigations of anemia, immune dysfunction, leukemia, and transplantation-related blood-production problems.