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Immunology and Infection
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Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
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Immunology and Infection
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JoVE Journal Immunology and Infection
Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

DOI:
10.3791/58061-v

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07:21 min

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July 29, 2018

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,
1Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center, 2Research Division of Immunology,Cedars-Sinai Medical Center

Chapters

  • 00:04Title
  • 00:32Magnetic-activated Cell Sorting (MACS) for Progenitor Enrichment
  • 02:06Fluorescence-activated Cell Sorting (FACS) for Myeloid Progenitor Identification and Isolation
  • 06:07Results: Representative Progenitor Yields and Purities
  • 06:45Conclusion

Summary

Automatic Translation

Automatic Translation

We demonstrate how to identify and isolate 6 subsets of myeloid progenitors from murine bone marrow using a combination of magnetic and fluorescence sorting (MACS and FACS). This protocol can be used for in vitro culture assays (methylcellulose or liquid cultures), in vivo adoptive transfer experiments, and RNA/protein analyses.

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Keywords: Myeloid ProgenitorsBone MarrowMACSLineage-negative CellsFACSNeutrophilsMonocytesDendritic CellsCell IsolationHematologyImmunology

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