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Isolation of Myoepithelial Cells from Adult Murine Lacrimal and Submandibular Glands
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Isolation of Myoepithelial Cells from Adult Murine Lacrimal and Submandibular Glands

Isolation of Myoepithelial Cells from Adult Murine Lacrimal and Submandibular Glands

DOI:
10.3791/59602-v

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07:15 min

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June 11, 2019

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, ,
1Department of Molecular Medicine,The Scripps Research Institute

Chapters

  • 00:04Title
  • 00:59Mouse Smooth Muscle Actin (SMA) Cell Labeling and Lacrimal Gland (LG) Harvesting and Microdissection
  • 02:10LG Single-cell Suspension Preparation
  • 04:46Antibody Staining and Fluorescence-activated Cell Sorting (FACS)
  • 05:45Results: Representative MEC and Pericyte Characterization and Isolation
  • 06:38Conclusion

Summary

Automatic Translation

Automatic Translation

The lacrimal gland (LG) has two cell types expressing α-smooth muscle actin (αSMA): myoepithelial cells (MECs) and pericytes. MECs are of ectodermal origin, found in many glandular tissues, while pericytes are vascular smooth muscle cells of endodermal origin. This protocol isolates MECs and pericytes from murine LGs.

Tags

Keywords: Myoepithelial CellsPericytesSmooth Muscle CellsExocrine GlandsTamoxifenLacrymal GlandParotid GlandCell IsolationCell LabelingCell SuspensionCell DigestionCell CultureGene Expression

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