Neonatal Chimera
Neonatal Chimera - Related Videos
07:37
CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
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2017
Here, we present chimera assembly by plasmid recovery and restriction enzyme site insertion (CAPRRESI), a protocol based on the insertion of restriction enzyme sites into synonym DNA sequences and functional plasmid recovery. This protocol is a fast and low-cost method for fusing protein-coding genes.
09:10
Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin
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Cited by 1 •
2013
Generation of lymph node/fat pad chimeras for the study of lymph node stromal cell origin is described. The method involves the isolation of lymph nodes from newborn mice and embryonic fat pads, the generation of chimeric lymph node-fat pads, and their transfer under the kidney capsule of a host mouse.
09:03
Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras
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Cited by 20 •
2010
Due to the hard chorion and soft embryos, manipulation of medaka embryos is more involved than in zebrafish. This video shows step-by-step procedures for how to manipulate medaka embryos, including dechorionation, mounting in agarose for imaging and cell transplantation for the production of chimeras. These procedures are essential to use medaka and zebrafish in a laboratory to take full advantage of their complementary features for the genetic dissection of vertebrate genome functions.
07:54
Isolation of Neonatal Extrahepatic Cholangiocytes
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Cited by 17 •
2014
A technique to isolate cholangiocytes from the extrahepatic bile ducts of neonatal mice is described. The ducts are meticulously dissected, and then cells are isolated by outgrowth in thick collagen gels. This method provides a useful tool for studying extrahepatic bile duct development and pathology.
09:38
Assessing Species-specific Contributions To Craniofacial Development Using Quail-duck Chimeras
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Cited by 21 •
2014
This article describes a method to generate chimeric embryos that are designed to test the species-specific contributions of neural crest and/or other tissues to craniofacial development.
FAQs
Two routes are highlighted. Researchers can aggregate early embryos, allowing cell populations to develop together, or introduce donor pluripotent cells into a host embryo. Comparing these strategies helps investigators examine how the starting arrangement of genetically distinct cells affects their later contributions to tissues. Both approaches depend on integration during development.
Developmental integration places donor and host cells in the same organism while tissues form. Their shared setting lets researchers compare cell fate, tissue contribution, and gene-function effects within the developing body. It also supports analysis of interactions between genetically distinct populations, including their relationships with the immune system.
Researchers distinguish the populations through genetic differences or molecular markers. These labels allow them to track cellular origin across tissues and compare where each population contributes. Linking origin with tissue structure and physiological outcomes helps connect early developmental history to the organization and performance of the neonatal organism.
A typical workflow begins with early embryos or donor pluripotent cells. Researchers either aggregate the embryos or introduce donor cells into a host embryo, then allow development to proceed. At the neonatal stage, they examine tissues and physiological outcomes while using genetic or molecular markers to determine the contributions of each cell population.
These experiments can address cell fate, tissue development, gene function, immune-system interactions, and developmental disorders. Because genetically distinct populations develop within one body, researchers can relate specific cellular contributions to tissue structure and physiological outcomes. This makes the approach useful for connecting developmental processes with later biological characteristics.
The model allows researchers to examine how genetically distinct cell populations contribute to developing tissues and how those contributions relate to physiological outcomes. By tracking cellular origin and evaluating gene function, investigators can study developmental differences within an organism rather than only in isolated populations. This provides biological context for investigating developmental disorders.