Objective Lens
Objective Lens - Related Videos
06:23
Using Baseplating and a Miniscope Preanchored with an Objective Lens for Calcium Transient Research in Mice
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Cited by 4 •
2021
The shrinkage of dental cement during curing displaces the baseplate. This protocol minimizes the problem by creating an initial foundation of the dental cement that leaves space to cement the baseplate. Weeks later, the baseplate can be cemented in position on this scaffold using little new cement, therebyreducing shrinkage.
03:14
Eye Lens Dissection: A Technique to Observe Zebrafish Lens Morphology
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2023
This video describes the protocol which is developed to dissect adult and larval zebrafish lenses to analyze cortical lens morphology and structural integrity of the lens sutures.
14:38
Creating Objects and Object Categories for Studying Perception and Perceptual Learning
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Cited by 12 •
2012
We describe a novel methodology for creating naturalistic 3-D objects and object categories with precisely defined feature variations. We use simulations of the biological processes of morphogenesis and phylogenesis to create novel, naturalistic virtual 3-D objects and object categories that can then be rendered as visual images or haptic objects.
05:14
Simulating the Mechanics of Lens Accommodation via a Manual Lens Stretcher
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Cited by 9 •
2018
We present an efficient method of studying lens accommodation by using a manual lens stretcher. The protocol mimics physiological accommodation by pulling the zonules connected around the lens capsule, thereby, stretching the lens.
10:14
Object Substitution Masking
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2023
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University Visual masking is a term used by perceptual scientists to refer to a wide range of phenomena in which in an image is presented but not perceived by an observer because of the presentation of a second image. There are several different kinds of masking, many of them relatively intuitive and unsurprising. But one surprising and important type of masking is called Object Substitution Masking. It has been a focus of research in vision...
FAQs
Numerical aperture controls two linked aspects of objective performance: the amount of light collected from a specimen and the fineness of spatial detail that can be resolved. An objective with a suitable numerical aperture can therefore improve light collection while distinguishing nearby biological structures, which is essential for interpreting small features in cells, tissues, and microorganisms.
Immersion objectives use oil or water to improve light collection during imaging. This property can be especially relevant when specimens contain fine structures or when labeled features must be observed clearly. Choosing an immersion objective therefore connects the optical conditions of the observation with the need to collect sufficient light from the biological sample.
Light collection and spatial resolution describe related but distinct aspects of objective performance. Collecting more light affects how much optical information enters the system, whereas resolution concerns how finely nearby details can be distinguished. Considering both helps researchers match an objective to observations requiring either strong light gathering, fine detail, or both.
Objective lenses support imaging across a broad range of biological specimens, including cells, tissues, and microorganisms. Their optical performance determines how effectively features within these samples can be observed and distinguished. This makes them useful for examining structure at different biological scales rather than limiting microscopy to one specimen type.
In fluorescence microscopy, the objective lens enables observation of fluorescent labels associated with biological samples. These observations can reveal where labeled molecules are localized within cells or tissues and can also support examination of changing cellular processes. The lens therefore contributes to both spatial information and the study of cellular behavior over time.
Objective-lens imaging can support measurements of morphology, molecular localization, and dynamic cellular processes. Morphology concerns the form and structure of biological specimens, while molecular localization identifies where labeled or otherwise observable molecules are positioned. Together, these outputs allow microscopy to connect cellular appearance with molecular distribution and changing activity.