Olfactory Neurons

Olfactory neurons are specialized sensory nerve cells that detect airborne odor molecules and convert chemical information into neural signals, forming the first stage of smell perception. Located in the olfactory epithelium, their receptor proteins bind specific odorants, triggering electrical activity that travels through the olfactory nerve to the brain’s olfactory bulb, where odor patterns are processed. In biology, these neurons provide a model for studying sensory transduction, neuronal regeneration, and connections between smell, memory, and behavior. Research on olfactory neurons also supports investigations of environmental sensing, neurological disease, and potential biomarkers of changes in brain function.

Olfactory Neurons - Related Videos

Research

JoVE Journal - Neuroscience

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

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Cited by 7 •

2014

Herein we describe the process of whole mount immunostaining of Drosophila antennae, which enables us to better understand the molecular mechanisms involved in the diversification of olfactory receptor neurons (ORN)s.

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

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Cited by 7 •

2011

We present a lentiviral technique for genetic manipulation and visualization of single olfactory sensory neuron axon and its terminal arborization in vivo.

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons

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Cited by 8 •

2011

Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds. Here, we show how to obtain patch-clamp recordings combined with photolysis of caged cAMP or caged Ca for the study of olfactory transduction in dissociated mouse olfactory sensory neurons.

Perforated Patch-Clamp Recording of Olfactory Sensory Neurons in Mice

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2025

This video demonstrates electrophysiological recording of olfactory sensory neurons (OSNs) in mice using a perforated patch-clamp technique. The olfactory epithelium, containing OSNs with fluorophore-tagged odor receptors, is isolated from a mouse and secured in a recording chamber. A recording pipette is clamped to the dendritic knob of an OSN, and nystatin within the pipette perforates the membrane to form electrical connectivity with the cell. Finally, another pipette is used to deliver an...

Coculturing of Retinal Ganglion Neurons with Olfactory Ensheathing Glia

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2025

This video demonstrates the procedure to isolate retinal ganglion neurons (RGNs) from a rat's retina and coculture them with olfactory ensheathing glia (OEG) cells. This method allows for studying the neuro-regenerative potential of OEGs after neural injury.

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