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Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
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JoVE Journal Neuroscience
Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons

DOI:
10.3791/3195-v

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11:35 min

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October 29, 2011

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, ,
1SISSA,International School for Advanced Studies, 2Istituto di Biofisica,Consiglio Nazionale delle Ricerche, 3SISSA Unit,Italian Institute of Technology

Chapters

  • 00:05Title
  • 00:08Introduction
  • 01:35Instrumentation
  • 03:37Dissociation of Olfactory Sensory Neurons
  • 07:51Recording
  • 09:37Representative Results
  • 11:09Conclusion

Summary

Automatic Translation

Automatic Translation

Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds. Here, we show how to obtain patch-clamp recordings combined with photolysis of caged cAMP or caged Ca for the study of olfactory transduction in dissociated mouse olfactory sensory neurons.

Tags

Flash PhotolysisCaged CompoundsOlfactory Sensory NeuronsPatch-clamp RecordingsPhotolysis Of Caged CompoundsOlfactory TransductionDissociated Mouse Olfactory Sensory NeuronsCiliaOdorant BindingReceptorsCAMPCyclic Nucleotide-gated (CNG) ChannelsCa EntryCa-activated Cl ChannelsMouse Olfactory EpitheliumFlash LampUltraviolet FlashesWhole-cell Voltage-clamp Configuration

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