Organ-on-chip
Organ-on-chip - Related Videos
01:35
Organ-on-Chip Models
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2026
Introduction to Organ-on-Chip Models Organ-on-chip models are advanced microphysiological systems that recreate key structural and functional features of human organs in a controlled laboratory setting. These devices integrate living human cells within microfluidic channels, allowing researchers to simulate tissue–tissue interfaces, fluid flow, and mechanical forces that are essential for normal organ function. Unlike traditional cell cultures, which often lack these dynamic physiological cues,...
06:12
Modeling the Endothelial Glycocalyx Post-Pneumonectomy in a 3D Fluidic Chip - An Approach to Fabricating a Vascular-based Organ-on-Chip System
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2025
Here we present a protocol for constructing an organ-on-chip (OOC) system designed to mimic pneumonectomy. This system investigates the potential correlation between glycocalyx impairment and complications following pneumonectomy.
10:51
Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance
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Cited by 41 •
2017
This publication describes the fabrication of an organ-on-chip device with integrated electrodes for direct quantification of transendothelial electrical resistance (TEER). For validation, the blood-brain barrier was mimicked inside this microfluidic device and its barrier function was monitored. The presented methods for electrode integration and direct TEER quantification are generally applicable.
09:46
Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip
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Cited by 2 •
2023
The present protocol describes the capabilities and the essential culture modalities of the Open-Top Organ-Chip for the successful establishment and maturation of full-thickness organ-on-chip cultures of primary tissues (skin, alveolus, airway, and intestine), providing the opportunity to investigate different functional aspects of the human epithelial/mesenchymal and vascular niche interface in vitro.
14:44
Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips
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Cited by 43 •
2018
Here, we present a protocol that describes the fabrication of stretchable, dual channel, organ chip microfluidic cell culture devices for recapitulating organ-level functionality in vitro.
FAQs
Microfluidic channels regulate fluid flow around living cells, allowing researchers to control exposure to chemical signals and tissue-specific mechanical forces. These conditions can help preserve physiologically relevant behavior and reproduce interfaces found in organs. As a result, experiments can examine how cells respond under controlled physical and chemical environments rather than in static culture alone.
Mechanical forces and fluid movement provide environmental cues that influence how cells function and interact. Organ-on-chip systems make those cues experimentally controllable, helping investigators study tissue responses under conditions that more closely reflect human biology. This is particularly relevant when examining barrier function, inflammation, tissue interactions, or responses to chemical treatments.
Conventional cell cultures may not reproduce the combined physical, chemical, and tissue-interface conditions present in an organ. Organ-on-chip models add controlled fluid flow, mechanical forces, and engineered interfaces around living cells. This broader set of conditions can support more physiologically relevant investigation of tissue behavior, disease processes, toxicity, and drug responses.
These models can be used to investigate barrier function, interactions between tissues, inflammation, and cellular responses to drugs or other chemical exposures. By controlling the surrounding laboratory conditions, researchers can isolate and examine particular biological processes. The resulting observations support studies of normal human biology as well as disease-related changes.
A study begins by establishing living cells within a device designed with relevant channels and interfaces. Researchers then control fluid flow and expose the cells to selected mechanical or chemical conditions while observing the resulting biological response. This workflow enables targeted investigation of tissue behavior, inflammation, barrier properties, disease mechanisms, or treatment effects.
Researchers use these models when they need controlled human-cell systems for disease modeling, toxicity testing, or pharmacological research. The devices allow responses to chemical treatments and tissue interactions to be examined under physiologically relevant conditions. They can therefore complement conventional culture approaches and help investigate how biological systems respond to potential therapies or harmful exposures.
Organ-on-chip systems can contribute to personalized medicine by providing a controlled setting for investigating human biological responses relevant to an individual research or treatment context. Their ability to model tissue behavior, disease processes, and drug responses supports comparison of biological reactions under defined conditions. This makes them useful for studying treatment effects alongside broader pharmacological research.
Organ-on-chip models offer a controlled platform for investigating human biology, disease processes, toxicity, and drug responses using living cells in engineered devices. Because they can reproduce selected tissue features and responses, they may reduce reliance on animal studies while enabling focused experiments. Their value lies in complementing existing research approaches, not simply replacing every biological model.