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Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips
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Bioengineering
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JoVE Journal Bioengineering
Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips

Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips

DOI:
10.3791/58151-v

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14:44 min

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October 20, 2018

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, , , , , , , , , ,
1Wyss Institute for Biologically Inspired Engineering,Harvard University, 2Apple, Inc, 3Harvard John A. Paulson School of Engineering and Applied Sciences,Harvard University, 4Vascular Biology Program and Department of Surgery,Boston Children’s Hospital and Harvard Medical School

Chapters

  • 00:00Title
  • 01:20Top Channel Preparation
  • 03:55Bottom Channel Preparation
  • 05:50PDMS Membrane Preparation
  • 09:27Top Assembly and Preparation
  • 11:02Chip Assembly
  • 11:56Results and Referenced Figures
  • 14:25Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol that describes the fabrication of stretchable, dual channel, organ chip microfluidic cell culture devices for recapitulating organ-level functionality in vitro.

Tags

Keywords: Microfluidic Organ Chips3D Printed MoldsSoft Silicone RubberMechanical CuesPerfusionIn Vitro Organ FunctionBridging Animal Studies And Clinical TrialsPDMS FabricationPolyurethane MoldsVacuum DesiccationOven CuringTile Scraper Cutting

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