Journal
/
Biology
/
Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

DOI:
10.3791/52824-v

•

07:48 min

•

July 03, 2015

•

,
1Department of Anesthesiology and Perioperative Care,University of California Irvine, 2Department of Biomedical and Molecular Sciences,Queen’s University, 3Department of Pharmacology,University of California Irvine

Chapters

  • 00:05Title
  • 01:25Drug Treatment of Cultured Cells and Immunocytochemistry
  • 03:26Microscope Settings and Imaging
  • 04:57Colocalization Analysis and Data Normalization
  • 06:17Results: Representative Photomicrographs of Receptor and Compartment-marker Labeling with False-color Colocalization Maps
  • 07:18Conclusion

Summary

Automatic Translation

Automatic Translation

Receptor trafficking modulates signaling and cell responsiveness to ligands and is, itself, responsive to cell conditions, including ligand-induced signaling. Here, we describe a powerful and flexible technique for quantitatively assessing drug-induced receptor trafficking using immunolabeling and colocalizational analysis.

Tags

Keywords: Receptor TraffickingReceptor InternalizationColocalization AnalysisImmunolabelingConfocal MicroscopyDrug-induced ChangesCell SignalingIntracellular TraffickingQuantitative Analysis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52824?article_type=v

Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging
check_url/52824?article_type=v

Environmentally Induced Heritable Changes in Flax
check_url/52824?article_type=v

Monitoring Equilibrium Changes in RNA Structure by ‘Peroxidative’ and ‘Oxidative’ Hydroxyl Radical Footprinting
check_url/52824?article_type=v

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
check_url/52824?article_type=v

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
check_url/52824?article_type=v

Analysis of SCAP N-glycosylation and Trafficking in Human Cells
check_url/52824?article_type=v

Live Cell Imaging and 3D Analysis of Angiotensin Receptor Type 1a Trafficking in Transfected Human Embryonic Kidney Cells Using Confocal Microscopy
check_url/52824?article_type=v

Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy
check_url/52824?article_type=v

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
check_url/52824?article_type=v

Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy

Read Article