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Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
JoVE Journal
Biology
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JoVE Journal Biology
Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

DOI:
10.3791/55637-v

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11:39 min

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July 21, 2017

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1Amnis Biology Department,MilliporeSigma

Chapters

  • 00:05Title
  • 00:42Jurkat Cell LC3, LAMP1, and p62 Labeling
  • 02:18Multispectral Imaging Flow Cytometry (MIFC)
  • 03:43MIFC Analysis
  • 08:35Results: Representative MIFC Analyses
  • 10:28Conclusion

Summary

Automatic Translation

Automatic Translation

Here, multispectral imaging flow cytometry with an analytical feature that compares bright detail images of 3 autophagy markers and quantifies their co-localization, along with LC3 spot counting, was used to measure autophagy in an objective, quantitative, and statistically robust manner.

Tags

Keywords: AutophagyLC3P62LAMP1ColocalizationMultispectral Imaging Flow CytometryQuantificationJurkat CellsFormalinPermeabilizationDAPILaser PowerDot PlotCell Population

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