Plant Microbiome Profiling

Plant microbiome profiling is the systematic characterization of microorganisms living on and within plants, providing insight into how microbial communities influence plant biology and ecosystem function. Researchers collect plant-associated samples, extract microbial DNA, and use marker-gene sequencing or metagenomics to identify community members, estimate their relative abundance, and compare microbial diversity across tissues, environments, or growth conditions. In biology, these profiles help link bacteria, fungi, and other microbes to nutrient cycling, plant growth, stress tolerance, and disease suppression. The resulting data support research on plant health, sustainable agriculture, host-microbe interactions, and strategies for managing beneficial microbial communities.

Plant Microbiome Profiling - Related Videos

Research

JoVE Journal - Biology

An Easy Method for Plant Polysome Profiling

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Cited by 20 •

2016

This protocol describes an easy method to extract and fractionate transcripts from plant tissues on the basis of the number of bound ribosomes. It allows a global estimate of translation activity and the determination of the translational status of specific mRNAs.

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

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Cited by 26 •

2012

A technique called Comprehensive Microarray Polymer Profiling (CoMPP) for the characterisation of plant cell wall glycans is described. This method combines the specificity of monoclonal antibodies directed to defined glycan-epitopes with a miniature microarray analytical platform allowing screening of glycan occurrence in a broad range of biological contexts.

Research

JoVE Journal - Biology
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OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides

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Cited by 35 •

2010

A rapid way is described to gain insights into the structure of polysaccharides in an extracellular matrix. The method takes advantage of the specificity of glycosylhydrolases and the sensitivity of mass spectrometry allowing minute amounts of materials to be analyzed. This technique is adaptable to be used directly on tissue itself.

Research

JoVE Journal - Biology
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Generation of Composite Plants in Medicago truncatula used for Nodulation Assays

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Cited by 7 •

2011

We demonstrate how hairy root composite plants can be used to study plant-rhizobium interactions and nodulation in the difficult-to-transform species Medicago truncatula.

Research

JoVE Journal - Biology
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Transmitting Plant Viruses Using Whiteflies

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Cited by 41 •

2013

Improving our understanding of and our ability to manage many of the insect-transmitted plant viruses requires the use of the vector. Insect transmission of plant viruses is a tritrophic interaction, and as such requires the manipulation of insects, virus, and plant. Vectors must be reared in large numbers and manipulated in such a way as to insure high rates of transmission to test plants. The basics of rearing and manipulating the whitefly, Bemisa tabaci (Hemiptera: Aleyrodidae), a vector of...

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