Platelet Population
Platelet Population - Related Videos
09:13
Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
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Cited by 5 •
2017
We describe a straightforward method for the isolation of washed platelets from human blood followed by agonist-induced platelet aggregation measurements by turbidimetry. As an example we apply this method for studying the aggregation response of human platelets to collagen after a pre-incubation with the Pannexin1 channel inhibitor Brilliant Blue FCF.
10:08
Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets
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Cited by 2 •
2024
This article outlines the protocol for quantifying the percentage of Tissue Factor (TF)-positive platelets using whole blood flow cytometry, assessing the protein: (1) intracellularly in resting conditions, and (2) on the cell surface, in both resting and activated conditions. Guidance is also provided for evaluating TF-positive platelets in platelet-rich plasma.
03:58
Purification and Activation of Platelets from Murine Whole Blood
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2025
In this video, we describe a protocol for purifying platelets from murine whole blood and their subsequent activation with the platelet agonist thrombin. When analyzed with flow cytometry, the purified resting platelets are characterized based on the expression of their cell-surface marker CD41, whereas purified activated platelets co-express both the surface markers CD41 and CD62P.
11:40
Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets
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Cited by 74 •
2014
Blood leukocytes and platelets can be used as a marker of overall bioenergetic health of an individual and so have the potential to monitor pathological processes and the impact of treatments. Here we describe a method to isolate and measure mitochondrial function and the oxidative burst in these cells.
09:38
A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
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Cited by 13 •
2017
This paper describes an experimental model of hemostasis that simultaneously measures platelet function and coagulation. Platelet and fibrin fluorescence is measured in real-time, and platelet adhesion rate, coagulation rate, and onset of coagulation are determined. The model is used to determine platelet procoagulant properties under flow in concentrates for transfusion.
FAQs
These measurements provide complementary information rather than relying on platelet number alone. The count describes how many platelets circulate, while characteristics and distribution add information about the population being measured. Together, they help relate circulating platelets to hemostatic capacity and vascular health, supporting a broader interpretation of blood status than any single platelet measurement can provide.
Bone marrow megakaryocytes release anucleate platelet fragments into the circulation, establishing the population available to respond to vascular injury. This production step connects the observed blood population with cellular activity in the marrow. Studying that connection helps biology researchers examine how platelet production and platelet function may relate to disease mechanisms.
A platelet count measures the number of circulating platelets, whereas a size distribution describes how platelet sizes are represented within that population. Including both perspectives can reveal population characteristics that a total count does not show by itself. This combined view supports more informative analyses of platelet populations and their relationship to hemostatic capacity.
Assessment uses platelet counts, size distributions, and related blood analyses to characterize the circulating population. The count addresses quantity, while size-related measurements describe population features. Interpreting these results together can support evaluation of bleeding and clotting disorders, inflammation, vascular health, and responses to treatment without reducing the analysis to a single numerical value.
These measurements are relevant when investigators or clinicians evaluate bleeding and clotting disorders, inflammation, or treatment responses. They can also contribute to assessment of vascular health by connecting the observed platelet population with hemostatic capacity. Their value comes from placing platelet results within a broader biological or biomedical question rather than viewing them in isolation.
In biology and biomedical research, population measurements help connect platelet production and function with disease mechanisms. Researchers can compare the number and characteristics of circulating platelets with conditions involving hemostasis, vascular health, inflammation, or treatment response. This approach provides a measurable link between cellular processes in blood and broader biological changes associated with disease.